Bacillus azotoformans
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus azotoformans  Pichinoty, de Barjac,
Mandel and Asselineau 1983.
Gram-negative or positive rods, 0.5-0.8 x 3.0-7.0 µm, motile by means of peritrichous
flagella. Terminal or subterminal  ellipsoidal endospores (1.4 x 2-2.5 µm), swelling
the sporangia.
Colonies are partially translucent, with entire margins, up to 5 mm in diameter on
yeast extract agar. Growth requirements are complex; growth factors are required.
Aerobic. Growth is observed at pH 6 - 7; temperature range 30 to 42-46 ºC. NaCl is
not required for growth; no growth in 5-7% NaCl. Allantoin or urate are not required.
Isolated from garden soil by enrichment culture in peptone broth in N2O atmosphere. No growth in the presence of 0.001% lysozyme.
Unknown.
  1. G. A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-
    127.
  2. Pichinoty F., de Barjac H., Mandel M. and Asselineau J., 1983. Description of Bacillus azotoformans sp. nov. IJSB 33, 3, 660-662.
Positive results for oxidase, nitrate reduction & citrate utilization.

Negative results for arginine dihydrolase, catalase, hydrolysis of Tween 80, degradation of tyrosine, deamination of phenylalanine,
beta-galactosidase, hydrolysis of esculin, hydrolysis of urea, hydrolysis of hippurate, hydrolysis of gelatin, egg yolk reaction, hydrolysis
of starch, Voges-Proskauer test, acid production from: D-glucose, D-mannose, fructose, galactose, lactose, maltose, ribose, sorbose
& D-xylose.
(c) Costin Stoica
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