Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Aerococcaceae, Genus Aerococcus, Aerococcus vaginalis Tohno et
Gram-positive coccoid cells, non-motile, grouped in pairs, tetrads or small groups.
Colonies are alpha-hemolytic, nonpigmented, circular, under 1 mm in diameter after
72 h on Columbia blood agar at 37 ºC. After 72 h of cultivation on MRS agar plates at
30 ºC, pinpoint colonies are circular, grey–white or white and under 0.5 mm diameter.
Facultatively anaerobic. Growth is inhibited by 2% oxgall. Growth occurs at 30–45 ºC,
pH 6.0–8.0 and in broth supplemented with 4% NaCl.
Isolated from the vaginal mucosa of a beef cow in Japan.
- Masanori Tohno, Maki Kitahara, Shuichi Matsuyama, Koji Kimura, Moriya Ohkuma, Kiyoshi Tajima: Aerococcus vaginalis sp.
nov., isolated from the vaginal mucosa of a beef cow, and emended descriptions of Aerococcus suis, Aerococcus viridans,
Aerococcus urinaeequi, Aerococcus urinaehominis, Aerococcus urinae, Aerococcus christensenii and Aerococcus sanguinicola.
Int J Syst Evol Microbiol, April 2014 64: 1229-1236.
Description is based on API 50 CH, API ZYM, API ID 32 Strep and API Strep results.
Positive results for alkaline phosphatase, hippurate hydrolysis, C4 esterase, leucine aminopeptidase, cystine aminopeptidase, acid
phosphatase, naphthol-ASBI-phosphohydrolase, beta-galactosidase, N-acetyl-beta-glucosaminidase, alanine-phenylalanine-proline
arylamidase, glycine-tryptophan arylamidase, acid production (after 7 days incubation at 30 ºC) from: N-acetylglucosamine,
D-fructose, D-galactose, D-glucose (without gas production), D-mannose, maltose, D-ribose & D-tagatose.
Negative results for arginine hydrolysis, acetoin production, aesculin hydrolysis, nitrate reduction, urease, C8 esterase, C14 lipase,
valine aminopeptidase, trypsin, chymotrypsin, alpha-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase,
alpha-mannosidase, alpha-fucosidase, pyrrolidonyl arylamidase, pyroglutamic acid arylamidase, beta-mannosidase, acid production
from: D-adonitol, aesculin ferric citrate, amygdalin, arbutin, D- and L-arabinose, D- and L-arabitol, cellobiose, dulcitol, erythritol, D-
and L-fucose, gentiobiose, gluconate, 2- and 5- ketogluconate, glycerol, glycogen, inositol, inulin, lactose, D-lyxose, D-mannitol,
melibiose, melezitose, methyl alpha-D-mannopyranoside, methyl alpha-D-glucopyranosidelactose, raffinose, L-rhamnose, salicin,
D-sorbitol, L-sorbose, starch, sucrose, trehalose, turanose, xylitol, methyl beta-D-xylopyranoside, D- and L-xylose.
(c) Costin Stoica