Chocolate agar
Sheep blood agar
QUALITY CONTROL
Following
microorganisms should have a good growth at 35 °C in 24 hours:
Neisseria meningitidis ATCC 13090,
Streptococcus pneumoniae ATCC 6305,
Streptococcus pyogenes ATCC 19615,
Bacillus cereus ATCC 11778.

Following germs should have a good growth at 28 °C in 3 days:
Candida albicans ATCC 10231,
Aspergillus niger ATCC 16404.
COMPOSITION

Nutrient agar composition:
Beef extract .......................................................................10g
Peptone .............................................................................10g
NaCl .....................................................................................5g
Agar ...................................................................................20g
Add distilled water 1000ml; adjust pH to 7.2 at 25 ºC. Sterilize by autoclaving at 121 ºC
for 15 minutes.

Melt nutrient agar base, cool to 45-50 ºC then add 5% sterile, defibrinated blood
(sheep, bovine or horse origin). For Haemophilus spp. and Neisseria spp.  prepare
chocolate agar by adding blood at higher temperature (70-80 ºC).
DESCRIPTION
Highly nutritive medium used for the isolation, cultivation and hemolytic ability testing of various fastidious microorganisms.
Blood agar
Sheep blood agar
Chocolate agar
(c) Costin Stoica
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