QUALITY CONTROL
Salmonella typhimurium ATCC 14028: good growth; red  (same colour as the medium / translucent) colonies with black center,
yellow colonies.
COMPOSITION
Yeast extract ................................................................................ 3.0 g
L-Lysine HCl ............................................................................... 5.0 g
Xylose ......................................................................................... 3.75 g
Lactose ........................................................................................ 7.5 g
Sucrose ........................................................................................ 7.5 g
Sodium desoxycholate .............................................................. 1.0 g
Sodium chloride ......................................................................... 5.0 g
Sodium thiosulphate ................................................................. 6.8 g
Ferric ammonium citrate .......................................................... 0.8 g
Phenol red ................................................................................ 0.08 g
Agar ............................................................................................ 13.5 g
Distilled water ad 1000ml; adjust pH to 7.4 ± 0.2. Do not overheat.
DESCRIPTION
Medium used for  isolation and identification of Salmonella and Shigella. H
2S-positive germs form black colonies. Fermentation of
xylose, lactose and sucrose results in yellow colonies, sometimes together with medium yellowing around colonies. Lysine
decarboxylation produces a purple colouration around the colonies.
Composition formula is included in SR EN ISO 6579 standard "Horizontal method for the detection of
Salmonella spp.".
XLD Agar
(Xylose, Lysine Deoxycholate agar)
(c) Costin Stoica
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