Distilled water ad 1000 ml, mix ingredients and heat to boiling max 1-2 min., adjust  pH 7.7, do not autoclave. Cool to 50 ºC and pour
PROCEDURE
Disperse culture or pathologic material on the medium surface, then incubate 48 hours at 37 ºC.

RESULTS
Salmonella strains  (excepting
S. paratyphi A & S. pullorum) form black-centre, metallic colonies.

REFERENCES
  1. Handbook Culture Media Merck, 1982.
  2. Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de microbiologie
    clinica ed. III, 2009, 1045-1084.
COMPOSITION
Meat extract .……………………………………….................………. 5.0 g
Peptone from meat ………………………………...…..............….. 10.0 g
D(+) glucose ....………………………………………….................… 5.0 g
Na
2HPO4 ....………………………………....................……………… 4.0 g
Iron(II) sulfate .…………………………………………..................…. 0.3 g
Brilliant green  …………………………………….….................… 0.025 g
Bismuth sulfite indicator ...………………………….................……. 8.0 g
Agar - agar .…………………………………………...............……..… 15 g
Distilled water ad 1000 ml, pH 7.6 ± 0.2, do not autoclave.
Pour in plates. Aspect: grayish yellow to pale green.

Wilson-Blair modified
Beef meat extract ……………………..……….................…………. 5.0 g
Gelatin pancreatic hydrolysate …………………….............…….. 10.0 g
Glucose ...………………………………………….................……… 5.0 g
Na
2HPO4 .....…………………………………..................…………… 4.0 g
Iron(II) sulfate …………………………………………..................…. 0.3 g
Brilliant green  …………………………………................….…… 0.025 g
Bismuth sulfite indicator ...……………………………................…. 8.0 g
Agar ..……..…………………………………………….....................… 20 g
DESCRIPTION
Selective medium for the isolation and differentiation of Salmonella from clinical specimens.
Bismuth-sulfite and brilliant green inhibit the associated microflora . H
2S-positive salmonellae form black colonies due to iron sulfide
formation. Reduction of bismuth ions to metallic bismuth produces the metallic look of the colonies.
Wilson Blair Medium
(Bismuth Sulfite Agar)
(c) Costin Stoica
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