RELATED MEDIA
Trypticase soy yeast extract medium
Trypticase Soy Broth (BD 211768) 30.0 g, Yeast extract 3.0 g, Agar 15.0 g, Distilled water ad 1000 ml.  Adjust for final pH 7.0 - 7.2.
Autoclave at 121 ºC for 15 minutes.
Tryptose agar
Tryptose 10 g, Meat extract 5 g, Yeast extract 5 g, Proteose peptone 10 g, Sodium chloride 5g, Agar 20 g, H2O ad 1000 ml, Final pH
7.2 at 25 ºC. May be supplemented with L-Cysteine HCl 0.25 g/l.
QUALITY CONTROL
Following germs should have a good growth at 35 °C in 24-48 hours:
Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 19615.
COMPOSITION
Pancreatic digest of casein 15 g, Enzymatic digest of soya bean 10 g, NaCl 5 g, Agar
15 g, Distilled water ad 1000ml; adjust pH to 7.3 ± 0.2.
Sterilize by autoclaving at 121 ºC for 15 minutes.

Medium may also be used as a blood agar base. Melt, cool to 45-50 ºC then add 5%
sheep or bovine blood.
DESCRIPTION
Medium is used for the growth of a large variety of microorganisms. May also be used for colony counting.
Tryptone Soya Agar
(Casein soya bean digest agar)
(c) Costin Stoica
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