RELATED MEDIA

Trypticase soy yeast extract medium
Trypticase Soy Broth (BD 211768) ................................30.0 g
Yeast extract ....................................................................... 3.0 g
Agar ....................................................................................15.0 g
Distilled water ad 1000 ml.  Adjust for final pH 7.0 - 7.2. Autoclave at 121°C for 15 minutes.

Tryptose agar
Tryptose ............................................................................... 10g
Meat extract ........................................................................... 5g
Yeast extract .......................................................................... 5g
Proteose peptone .............................................................. 10g
Sodium chloride ................................................................... 5g
Agar ...................................................................................... 20g
H
2O ad 1000 ml, Final pH 7.2 at 25°C
May be supplemented with
L-Cysteine HCl ..............................................................0.25g/l
QUALITY CONTROL
Following germs should have a good growth at 35 °C in 24-48 hours:
Staphylococcus aureus ATCC 25923, Streptococcus pyogenes
ATCC 19615
COMPOSITION
Pancreatic digest of casein ..................................................................15g
Enzymatic digest of soya bean .............................................................10g
NaCl .......................................................................................................5g
Agar .....................................................................................................15g
Distilled water ad1000ml; adjust pH to 7.3 ± 0.2 Sterilize by autoclaving at 121 ºC for
15 minutes.

Medium may also be used as a blood agar base. Melt, cool to 45-50 ºC then add 5%
sheep or bovine blood.
DESCRIPTION
Medium  used for the growth of a large variety of microorganisms. May also be used for colony counting.
Tryptone Soya Agar
(Casein soya bean digest agar)
(c) Costin Stoica
Antibiogram
Encyclopedia
Culture media
Biochemical tests
Stainings
Images
Movies
Articles
Identification
Software
R E G N U M
PROKARYOTAE
Previous page
Back