QUALITY CONTROL
Brochothrix thermosphacta ATCC 11509 - good / luxuriant growth,
Escherichia coli ATCC 25922  - inhibited,
Enterococcus faecalis ATCC 29212  - inhibited,
Staphylococcus aureus ATCC 25923 - inhibited,
Pseudomonas aeruginosa ATCC 27853 - poor growth / none,
Salmonella Enteritidis ATCC 13076 - inhibited,
Canida albicans ATCC 10231- poor growth / none.

REFERENCES
1. Sneath P.H.A., 2009. Genus II. Brochothrix Sneath and Jones 1976. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig,
F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 257-262.
2. HiMedia Lab. S.T.A. Agar Base M1299 Technical data. Revision : 1 / 2011.
COMPOSITION
Glycerol nutrient agar: peptone (Oxoid), 20 g; yeast extract (Oxoid), 2 g; glycerol,15 g; K2HPO4, 1 g; MgSO4·7H2O, 1 g; agar (Oxoid No.
3), 13 g; distilled water, 1000 ml; pH 7.0.
Autoclave 121°C for 15 minutes.

STAA: to the molten glycerol nutrient agar add the following solutions in sterile distilled water: streptomycin sulfate (Glaxo) to a final
concentration of 500 μg/ml, actidione (Upjohn) to 50 μg/ml, thallous acetate to 50 μg/
ml. Mix well and dispense.
DESCRIPTION
STAA (Streptomycin Thallous Acetate Agar) is recommended for the isolation of
Brochothrix thermosphacta from foods. Brochothrix
campestris
can also be isolated. Lactobacillus, Listeria, Erysipelothrix, Streptococcus, and Bacillus and coryneform bacteria do not
grow on STAA medium.
STAA & Glycerol Agar
(c) Costin Stoica
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