MRS Agar
OTHER MEDIA
Rogosa selective Lactobacillus agar (composition per liter)
Agar ................................................................................15.0 g
Sodium acetate............................................................ 15.0 g
Glucose .........................................................................10.0 g
Casein (digested) .......................................................10.0 g
K
2HPO4 .............................................................................6.0 g
Yeast extract ................................................................... 5.0 g
Arabinose ....................................................................... 5.0 g
Saccharose .................................................................... 5.0 g
Ammonium citrate ......................................................... 2.0 g
Sorbitan monooleate .................................................... 1.0 g
MgSO
4 *7H2O ............................................................... 0.57 g
MnSO4.7H2O ................................................................0.12 g
FeSO4.H2O .................................................................. 0.03 g
Acetic acid (glacial) ................................................... 1.32 ml
Streptococcus equinus & Streptococcus gallolyticus selective medium.
QUALITY CONTROL
Incubate 3 days at 35 °C or  5 days at 30 °C, better in CO2 enriched atmosphere.
Lactobacillus gasseri ATCC 19992:  good growth; yellow colonies.
Escherichia coli ATCC 25922: no growth.
Bacillus cereus ATCC 11778: no grow.
COMPOSITION
Peptone.............................................................................. 10g
Meat extract.......................................................................... 8g
Yeast extract........................................................................ 4g
Glucose.............................................................................. 20g
Sodium acetate 3H
2O........................................................ 5g
Tween 80............................................................................. 1g
Dipotassium hydrogen phosphate................................. 2g
Triammonium citrate......................................................... 2g
Magnesium sulfate 7H
2O ............................................. 0.2g
Manganese sulphate 4H
2O ....................................... 0.05g
Agar.................................................................................... 10g
H
2O ad 1000 ml, Final pH 6.2 at 25°C.

Sterilize by autoclaving at 118 ºC for 15 minutes. Aspect: medium to dark amber
coloured, clear to slightly opalescent.
DESCRIPTION
A special medium containing growth factors for lactic acid bacteria (
Lactobacillus spp. and related germs) from dairy products, foods,
feces and other sources.
MRS Agar
(de Man, Rogosa & Sharp)
(c) Costin Stoica
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