QUALITY CONTROL
Following germs should have a good to very good growth at 35-37 °C in 24
hours:
Escherichia coli (25922) and Escherichia coli (11775).
COMPOSITION
Contains per litre 10g tryptone (Difco), 5g yeast extract, 5g sodium chloride.
Supplements, such as 1% sorbitol, are added to Luria Bertani agar medium for
Erwinia amylovora and related species.

Adjust the pH of the solution to 7.4. For solid medium add 15 g agar to 1 litre of
Luria Bertani broth.

Sterilize by autoclaving at 121 ºC for 15 minutes.
DESCRIPTION
Medium used for cultivation  of Enterobacteriaceae (
Escherichia coli) and other organisms. It is nutritionally rich, suitable for the
growth of pure cultures like recombinant strains and for molecular genetic studies.
Luria Bertani medium
(c) Costin Stoica
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