Helicobacter pylori ATCC 43526 - good growth;
Candida albicans ATCC 10231 - inhibited or no growth.
Pancreatic digest of casein 10.0 g, meat peptic digest 5.0 g, Heart Pancreatic
Digest 3.0 g, Yeast Extract 5.0 g, Corn Starch 1.0 g, Sodium Chloride 5.0 g,
Supplements: Vitalex 10.0 ml, Amphotericin B 5 mg, Cefsulodin 0.005,
Trimethoprim 5 mg, Vancomycin 10 mg, lysed horse blood 70.0 ml, agar 14.0 g.
Final pH 7.3 ± 0.2.
Medium used for the isolation of Helicobacter pylori from clinical specimens (gastric biopsy).
Peptones provide nitrogen, carbon, amino acids and vitamins. Yeast extract and corn starch are included to supply a carbon energy
source in the form of B-complex vitamins. Vitalex and lysed horse blood supplements provide additional growth factors for fastidious
microorganisms. The antibiotics mix inhibits the contaminating flora without reducing the recovery of the target microorganism.
Cefsulodin and amphotericin B inhibits Candida spp.
(c) Costin Stoica
Because the microorganism requires microaerobic atmophere and high humidity,
inoculate the specimen as soon as posible after sample harvesting by streaking it
on the agar surface. Incubate plates in microaerobic atmosphere at 35-37 ºC for 3-5