Eosin Methylene Blue Agar
Escherichia coli on EMB Agar. Note the dark
colonies surrounded by a metallic-green halo
QUALITY CONTROL
Inoculate and incubate at 35 °C for 18-24 hours:
- Enterococcus faecalis ATCC 29212: partial inhibition.
- Escherichia coli ATCC 25922: good growth, black and green-metallic colonies due
to lactose fermentation.
- Proteus vulgaris ATCC 9484: good growth, colorless colonies
- Salmonella typhi ATCC 19430: good growth, colorless colonies
- Salmonella typhimurium ATCC: good growth, colorless colonies
COMPOSITION
Peptone from meat .............................................................10g
Bipotasic phosphate ............................................................2g
Lactose .................................................................................10g
Eosin G ................................................................................0.4g
Methylene blue ............................................................. 0.065g
Agar .......................................................................................15g
Distilled water ad 1000ml.
Autoclave at 121 °C for 15 minutes. Aspect: red-purple coloured, opalescent gel with
greenish cast .
DESCRIPTION
Selective and differential medium for the isolation, cultivation and differentiation of

Entrobacteriaceae
(coliforms) from foods, water, feces and pathologic materials.
The eosin and methylene blue inhibits most Gram-positive bacteria but allows
growing of many Gram-negative organisms.
Gram-negative bacteria that ferment the lactose produce acid which turns the
colonies dark purple as the acid acts upon the dyes. Strong lactose-fermenting
bacteria produce flat, dark colonies with a green metallic sheen.
Geam-Levine (EMB Agar Levine)
Eosin Methylene Blue Agar
GEAM Levine
(c) Costin Stoica
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