Thallium acetate  may be added to a max concentration of  1/10,000;
addition of pig serum to a final concentration of 20%; pH adjustment to 7.4

Composition of Hank's balanced salt solution (modified)
2 stock solutions needed:

Stock A
NaCl ................................................................................ 80 g
KCl ..................................................................................... 4 g
MgSO
4  7 H2O ................................................................... 1 g
MgCl
2 6 H2O ..................................................................... 1 g
dissolve in 400 ml water CaCl2 (anhydrous) 1.4 g; add water to 500 ml

Stock B
Na2HPO4 12 H2O .......................................................... 1.5 g dissolved in 400 ml water
KH
2PO4 .......................................................................... 0.6 g
water ad 500 ml
For use, add 25 ml of stock A to 400 ml of water, followed by 25 ml of stock B; then  water ad 500 ml.
COMPOSITION
Hank's balanced salt solution (modified)  ....................... 500 ml
Water ...................................................................................... 750 ml
Bacto brain heart infusin (Difco) ........................................... 8.2 g
Bacto PPLO broth w/o CV (Difco) ......................................... 8.7 g
Autoclaving at 121 'C for 2-5 minutes.

Yeast extract ............................................................................ 60 ml
0.5% phenol red solution .................................................... 4.5 ml
Bacitracin ............................................................................. 250 mg
Meticillin ............................................................................... 250 mg
DESCRIPTION
Medium is used for
Mycoplasma hyopneumoniae and Mycoplasma flocculare isolation.
Friis medium
(c) Costin Stoica
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