QUALITY CONTROL
Inoculate the medium and incubate for 36 hours at 35°C or for 3 days at 32°C.
Staphylococcus aureus ATCC 6538: good growth, yellow colonies surrounded by yellow areas.
Staphylococcus aureus ATCC 25923: good growth, yellow colonies surrounded by yellow areas.
Staphylococcus epidermidis ATCC 12228: no colour change, growth is usually poorer.
Escherichia coli ATCC 8739: no growth.
COMPOSITION
Proteose peptone .....................................................................10g
Beef extract ...................................................................................1g
NaCl ............................................................................................75g
D-Manite .....................................................................................10g
Phenol red ........................................................................... 0.025g
Agar .............................................................................................15g
Distilled water ad 1000ml; adjust pH to 7.4
Sterilize by autoclaving at 121 ºC for 15 minutes.
DESCRIPTION
bacteria are inhibited by the bile salts, the crystal violet and the high level of NaCl, with the exception of halophilic species. Medium is
also used for microbial contamination testing.
The correlation between pathogenicity and mannitol degradation to acid is not widely accepted.   
Mannitol Salt Agar
(MSA / Chapman agar)
Chapman
(c) Costin Stoica
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