QUALITY CONTROL
Following germs should have a good to very good growth at 35 °C in 24-48 hours:
Escherichia coli ATCC 25922, Staphylococcus
aureus
ATCC 25923, Streptococcus pneumoniae ATCC 6305, and Streptococcus pyrogenes ATCC 19615.
COMPOSITION
Casein peptone ....................................................................15g
Soymeal peptone ...................................................................5g
NaCl ..........................................................................................5g
Agar .........................................................................................15g

Adjust pH to 7.3,  distilled water ad 1000 ml. Sterilize by autoclaving at 121 ºC for 15
minutes.

For hemolysis test add 5% sheep or bovine blood.
DESCRIPTION
Medium used for cultivation, isolation of fastidious or nonfastidious microorganisms or for  the coliforms precultivation and
enumeration.
CASO agar
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