PROCEDURE
Disperse culture or pathologic material on the medium surface, then incubate 2-3 days at 37 ºC .

RESULTS
Bordetella colonies are smooth, raised, shiny, 1-2 mm in diameter, sometimes with a gray-black center or surrounded by hemolysis
zone.

REFERENCES
1. Handbook Culture Media Merck, 1982.
2. Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de microbiologie clinica
ed. III, 2009, 1045-1084.
COMPOSITION
Potatoes infusion …………………...………………………………..… 4.5 g
NaCl ………………………………………………………………….…... 5.5 g
Agar-agar ………………………………………………………………….15 g
Glycerol …………………………………………………….…………… 10 ml
Blood (sheep, rabbit or human) ………………………………
.150-200 ml
Penicillin ………………………………………………………….…… 250 IU
Distilled water ad 1000 ml, pH 6.6 ± 0.2, autoclave before adding blood, pour in plates. Plates can be stored refrigerated for 2 weeks.
Addition of 1% peptone (5 g/l peptone from casein & 5 g/l peptone from meat) without penicillin improves the growth of
Bordetella in
large vaccine cultures.

Potatoes infusion preparation: wash and peel the potatoes then slice them in small pieces. Add the NaCl  and potatoes in 500 ml
water. Boil 30 minutes. Filter then adjust pH to 7.0 .
DESCRIPTION
Medium was designed for the isolation of
Bordetella pertusis, Bordetella parapertussis and other Bordetella species.
Penicillin addition inhibits the growth of associate Gram-positive microflora.
Bordet – Gengou Agar Base
(Bordet & Gengou 1906 - modified)
(c) Costin Stoica
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