QUALITY CONTROL
Escherichia coli : intense turbidity and gas production.
Salmonella typhimurium: medium turbidity without gas production.
Proteus vulgaris: medium turbidity without gas production.
Staphylococcus aureus: no growth.
COMPOSITION
Peptic peptone ...............................................................................10g
Lactose ...........................................................................................
10g
Bile ...................................................................................................20g
Brilliant green .........................................................................0.0133g
Distilled water ad 1000 ml; adjust pH to 7.2.

This is the recipe for BBLV simple concentrated medium (Brilliant Green Bile Broth
2%).  For double concentrated BBLV (Brilliant Green Bile Broth 4%) double all
ingredients except water.
DESCRIPTION
Medium for detection and confirmation of coliform bacteria in foods, water and dairy products. Lactose fermentation with gas
production may be tested using Durham tubes. Recommended for the 44°C confirmatory test for
Escherichia coli.
BBLV (Brilliant Green Bile Lactose Broth)
BBLV
(c) Costin Stoica
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