PROCEDURE
Inoculate the culture medium  by streaking the plate. Incubate at 37 ºC for 2-7 days.

RESULTS
Blue medium => acetate positive; green medium => acetate negative.

REFERENCES
1. Costin I.D.: Utilization of sodium acetate by Shigella and Escherichia. J.Gen. Micrbiol., 41; 23-27, 1965
2. Trabulsi L.R. & Ewing W.H. : Sodium Acetate medium for the differentiation of Shigella and Escherichia cultures. Publ. Hlth. Lab.,
20; 137-140, 1962
3. Handbook Culture Media Merck, 1982.
COMPOSITION
Sodium chloride……………………………….....……. 5.0 g
Magnesium sulfate ………………………………....… 0.2 g
Ammonium dihydrogen phosphate ………...….…… 1.0 g
Di-potassium hydrogen phosphate ………………… 1.0 g
Sodium acetate ……
..……………………………...….. 2.0 g
Bromthymol blue ……
.....………………………....…. 0.08 g
Agar-agar …………………
..………………………..... 12.0 g (or 20 g in medium variant)

Distilled water ad 1000 ml, pH 7.0 ± 0.1, sterilize by autoclaving.
DESCRIPTION
Medium for the biochemical differentiation and identification of some Enterobacteriaceae on the basis of acetate degradation.
Degradation of the acetate results in an increase of the pH of the medium, changing colour to blue.
Acetate agar
(Trabulsi & Ewing 1962)
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