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(c) Costin Stoica
R E G N U M
PROKARYOTAE
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Bacterial Cultural Characteristics
Actinobacillus pleuropneumoniae  
dependent of NAD-producing
Staphylococcus aureus (yellow line)
Acinetobacter baumannii colonies on
sheep blood agar
Aerococcus viridans colonies on
sheep blood agar
Aeromonas hydrophila hemolytic
colonies on sheep blood agar
Alcaligenes faecalis colonies on
sheep blood agar
Avibacterium gallinarum colonies on
sheep blood agar
Agar pitting colonies (Bacillus spp.)
Bacillus amyloliquefaciens colonies
on Chapman agar
Bacillus atrophaeus brown-black
colonies on Mueller-Hinton agar
Bacillus anthracis colonies on sheep
blood agar
Bacillus anthracis culture on solid and
liquid media
Bacillus firmus colonies on sheep
blood agar
Bacillus licheniformis on sheep blood
agar
Bacillus megaterium colonies
Bacillus mycoides specific colony
shape
Bacillus pumilus on sheep blood agar
Bacillus subtilis culture on solid and
liquid media
Bacillus thuringiensis on sheep blood
agar
Bacteriophage - infected culture (spots
& plaques no/partial growth)
Bordetella bronchiseptica on blood
agar (left) and MacConkey agar(rigth)
Bordetella bronchiseptica on solid
medium
Campylobacter jejuni
Citrobacter braakii colonies on sheep
blood agar
Clostridium spp. on Sulphite agar
Clostridium spp. gas production &
turbidity in Liver Broth medium  
(positive-left and negative - right)
Corynebacterium amycolatum
colonies on sheep blood agar
Dendritiform and galaxy-shape
colonies of
Bacillus spp. Mixed culture
from animal feed
Enterobacter cloacae colonies on
sheep blood agar
Enterococcus faecalis colonies on
sheep blood agar
Enterococcus faecium on Bile-esculin
agar (dark brown halos)
Enterococcus faecium colonies on
sheep blood agar
Enterococcus faecium deep red
colonies on Slanetz Bartley agar
Enterococcus gallinarum colonies on
sheep blood agar
Erysipelothrix rhusiopathiae colonies
after 48h incubation
Erysipelothrix rhusiopathiae after 1
week anaerobic incubation
Escherichia coli beta haemolysis
on sheep blood agar
Escherichia coli on GEAM / Levine
medium (metallic look)
Escherichia coli on Rambach agar
(blue-green colonies)
Fusobacterium necrophorum
in thioglycollate broth
Granulicatella / Abiotrophia  adiacens
colonies, 24h-incubation
Helicobacter pylori colonies on sheep
blood agar
Hydrogen sulfide produced by
Salmonella on XLD agar (black)
Klebsiella pneumoniae (mucous,
confluent colonies)
Kocuria varians colonies on
sheep blood agar
Lactobacillus fermentum
colonies on MRS agar
Lactococcus lactis subsp. lactis on
sheep blood agar
Lactococcus garvieae on
sheep blood agar
Lactose-negative colonies on CLED
medium
Lactose-negative colonies on
Istrati-Meitert medium
Lactose-positive colonies on Mac
Conkey medium
Lactose-positive colonies on CLED
medium
Lysinibacillus sphaericus on sheep
blood agar
MacConkey agar: lactose+ (up), lactose-
(left) and lactose- but red pigmented
Serratia colonies (right)
Micrococcus spp. on solid medium
Moraxella lacunata colonies on sheep
blood agar
Mycobacterium spp. colonies on sheep
blood agar
Mycoplasma agalactiae colonies (B&W)
Mycoplasma agalactiae colonies,
magnification x70
Mycoplasma agalactiae broth culture
(positive - left and negative - right)
Nocardia asteroides - surface growth only
Paenibacillus macerans spreading
colonies on sheep blood agar
Pasteurella (Mannheimia) haemolytica
colonies on sheep blood agar
Pasteurella multocida colonies on Sheep
blood agar
Pasteurella multocida rising and twisting
deposit  when shaked
Proteus mirabilis swarming
Proteus mirabilis swarming and Dienes
effect
Pseudomonas aeruginosa, green,
fluorescent, diffusible pigment
Pseudomonas aeruginosa haemolysis on
Sheep blood agar
Pseudomonas aeruginosa on Milk agar
Pseudomonas stutzeri on sheep blood
agar
Salmonella spp. lactose-negative colonies
on MacConkey agar
Salmonella Gallinarum on MSRV medium
(non-motile)
Salmonella Enteritidis motility on MSRV
medium
Serratia marcescens red-pigmented
colonies after 72h incubation
Serratia rubidaea on milk agar
(prodigiosin pigment production)
Staphylococcus colony outskirts on sheep
blood agar. Hyperzoom 200x
Staphylococcus aureus carotenoid
pigment production after 48h
Staphylococcus aureus
alpha- & beta-hemolysis
Staphylococcus aureus shiny black
colonies on Baird-Parker agar
Staphylococcus chromogenes colonies on
sheep blood agar
Staphylococcus epidermidis colonies on
sheep blood agar
Staphylococcus haemolyticus colonies on
sheep blood agar
Staphylococcus xylosus colonies on
sheep blood agar
Streptococci haemolysis: alpha
haemolytic  (right),  beta hemolytic (left),
non-hemolytic (down)
Streptococcus agalactiae on sheep blood
agar (alpha-hemolytic)
Streptococcus agalactiae on sheep blood
agar (beta-hemolytic)
Streptococcus canis, strong beta-hemolytic
colonies on sheep blood agar
Streptococcus constellatus  colonies on
sheep blood agar
S. dysgalactiae subsp. dysgalactiae
(alpha-hemolytic, left) and
subsp.
equisimilis
(beta-hemolytic, right)
Streptococcus mitis colonies on sheep
blood agar
Streptococcus mutans colonies on BHI
agar
Streptococcus pyogenes colonies after
48h incubation
Streptococcus uberis colonies on sheep
blood agar