Phylum Proteobacteria, Class Gammaproteobacteria, Order Vibrionales, Family Vibrionaceae, Genus Vibrio, Vibrio splendidus
(Beijerinck 1900) Baumann, Baumann, Bang and Woolkalis 1981.
V. splendidus includes biotypes I, II and a unnamed biotype (Reichelt at al. 1976).
Historical synonyms: Photobacter splendidum Beijerinck 1900, Beneckea splendida
(Beijerinck 1900) Reichelt, Baumann and Baumann 1979.
Gram-negative straight or curved rods, motile. Not swarming on complex media.
Nonspore-forming. Bioluminescence is present in biotype I.
Grows at 18 -30 ºC. Weak or no growth at 4 and 35 ºC. Grows in media with 1 to 8%
NaCl. No growth in the absence of NaCl. Facultatively anaerobic.
Isolated from marine fish and seawater. Susceptible to vibriostatic compound 0/129.
May cause vibriosis in fish, oysters, mussels, and scallops.
Pathogenicity of V. splendidus biovar II was proved by experimental infection of Japanese oysters larvae.
- J.J. Farmer, M. Janda, 2004. Family I. Vibrionaceae. In: Bergey’s Manual of Systematic Bacteriology, Second edition,Vol two, part B,
George M. Garrity (Editor-in-Chief), pp. 491-546.
- J. G.Holt et al., 1994. Group 5 Facultatively anaerobic Gram-negative rods. Subgroup 2 Family Vibrionaceae. In: Begey’s Manual of
Determinative Bacteriology, 9-th edition, Williams & Wilkins. pp. 190-194.
- G. Sugumar, T. Nakai, Y. Hirata, D. Matsubara, K. Muroga: Vibrio splendidus biovar II as the causative agent of bacillary necrosis of
Japanese oysters Crassostrea gigas larvae. Dis Aquat Org vol.33: 111-118, 1998.
Positive results for nitrate reduction to nitrite, oxidase, lipase, indole production (Heart Infusion Broth, 1% NaCl) (biotype II is variable),
citrate utilization, arginine (1%NaCl) + (biotype II is negative), gelatin hydrolysis (1% NaCl, 22 °C), acid production from D-glucose,
cellobiose, glycerol, maltose, D-mannitol, D-mannose (biotype II is negative), sucrose (biotype II is negative) & trehalose.
Negative results for esculin hydrolysis, Voges-Proskauer (1% NaCl), urea hydrolysis, lysine (1% NaCl), ornithine (1% NaCl), gas
production from D-glucose, acid production from: D-adonitol, L-arabinose, D-arabitol, Dulcitol, D-galactose, myo-inositol, lactose,
melibiose, raffinose, L-rhamnose, salicin, D-sorbitol & D-xylose.
Variable results for ONPG test.
(c) Costin Stoica