Taxonomy
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Phylum Proteobacteria, Class Gammaproteobacteria, Order Vibrionales, Family Vibrionaceae, Genus Vibrio, Vibrio splendidus
(Beijerinck 1900) Baumann, Baumann, Bang and Woolkalis 1981.
V. splendidus includes biotypes I, II and a unnamed biotype (Reichelt at al. 1976).

Historical synonyms:
Photobacter splendidum Beijerinck 1900, Beneckea splendida
(Beijerinck 1900) Reichelt, Baumann and Baumann 1979.
Gram-negative straight or curved rods, motile. Not swarming on complex media.
Nonspore-forming. Bioluminescence is present in biotype I.
Grows at 18 -30 ºC. Weak or no growth at 4 and 35 ºC. Grows in media with 1 to 8%  
NaCl. No growth in the absence of NaCl. Facultatively anaerobic.
Isolated from marine fish and seawater. Susceptible to vibriostatic compound 0/129.
May cause vibriosis in fish, oysters, mussels, and scallops.
Pathogenicity of
V. splendidus  biovar II was proved by experimental infection of Japanese oysters larvae.
  1. J.J. Farmer, M. Janda, 2004. Family I. Vibrionaceae. In: Bergey’s Manual of Systematic Bacteriology, Second edition,Vol two, part B,
    George M. Garrity (Editor-in-Chief), pp. 491-546.
  2. J. G.Holt et al., 1994. Group 5 Facultatively anaerobic Gram-negative rods. Subgroup 2 Family Vibrionaceae. In: Begey’s Manual of
    Determinative Bacteriology, 9-th edition, Williams & Wilkins. pp. 190-194.
  3. G. Sugumar, T. Nakai, Y. Hirata, D. Matsubara, K. Muroga: Vibrio splendidus biovar II as the causative agent of bacillary necrosis of
    Japanese oysters Crassostrea gigas larvae. Dis Aquat Org vol.33: 111-118, 1998.
Positive results for nitrate reduction to nitrite, oxidase, lipase, indole production (Heart Infusion Broth, 1% NaCl) (biotype II is variable),
citrate utilization, arginine (1%NaCl) + (biotype II is negative), gelatin hydrolysis (1% NaCl, 22 °C), acid production from D-glucose,
cellobiose, glycerol, maltose, D-mannitol, D-mannose (biotype II is negative), sucrose (biotype II is negative) & trehalose.

Negative results for esculin hydrolysis, Voges-Proskauer (1% NaCl), urea hydrolysis, lysine (1% NaCl), ornithine (1% NaCl),  gas
production from D-glucose, acid production from: D-adonitol, L-arabinose, D-arabitol, Dulcitol, D-galactose, myo-inositol, lactose,
melibiose, raffinose, L-rhamnose, salicin, D-sorbitol & D-xylose.

Variable results for ONPG test.
(c) Costin Stoica
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