Phylum Proteobacteria, Class Gammaproteobacteria, Order Vibrionales, Family Vibrionaceae, Genus Enterovibrio, Enterovibrio
nigricans Pascual et al. 2009.
Gram-negative, motile rods or coccobacilli, 1.0-1.5 x 0.4-0.7 μm.
Chemo-organotrophic and facultatively anaerobic. Grows on seawater-based media
such as MA, on which some strains produce a black / deep-brown, slightly-diffusible
pigment. Colonies are round, with entire margins and a bright surface. Grows on TSA
supplemented with 1% NaCl. Does not swarm or luminesce. Growth on TCBS agar is
scarce and slow, producing green (non-sucrose-fermenting) colonies.
Growth is observed at 15–28 ºC but not at 4 or 37 ºC. Requires NaCl for growth. Media
containing 0.9–6.0% total salinity sustain good growth; no growth is observed at 0.4
or 7% or more salinity.
Isolated from the head kidney of Sparus aurata cultured on the Spanish Mediterranean coast.
- Javier Pascual, M. Carmen Macian, David R. Arahal, Esperanza Garay, and Maria J. Pujalte, Description of Enterovibrio nigricans
sp. nov., reclassification of Vibrio calviensis as Enterovibrio calviensis comb. nov. and emended description of the genus
Enterovibrio Thompson et al. 2002 Int J Syst Evol Microbiol July 2009 59:1850
Positive results for oxidase, Tween 80 hydrolysis, DNA-se, acid production from glucose (wthout gas production), D-fructose,
D-galactose, lactose, melibiose, D-mannitol and D-sorbitol.
Can utilize D-glucose, D-mannose, N-acetyl-D-glucosamine, D-gluconate & L-glutamate.
Negative results for casein hydrolysis, gelatin hydrolysis, starch hydrolysis, alginate hydrolysis, lecithinase, lysine decarboxylase,
ornithine decarboxylase, indole production, Voges-Proskauer test, acid production from D-ribose, D-xylose, L-arabinose, trehalose,
L-rhamnose, maltose, cellobiose, amygdalin, salicin & myo-inositol.
Cannot utilize L-arabinose, D-xylose, trehalose, D-galactose, L-rhamnose, maltose, cellobiose, sucrose, lactose, melibiose, salicin,
amygdalin, D-mannitol, D-sorbitol, myo-inositol, D-glycerate, D-saccharate, D-glucuronate, D-galacturonate, pyruvate, propionate,
butyrate, citrate, trans-aconitate, 2-oxoglutarate, succinate, malate, 3-hydroxybutyrate, glycine, L-leucine, L-serine, L-threonine,
L-alanine, L-arginine, L-tyrosine, L-ornithine, L-citrulline, L-aspartate, L-histidine, L-lysine, L-sarcosine & putrescine.
Variable results for nitrate reduction to nitrite (not to N2), arginine dihydrolase, utilization of: D-ribose, D-fructose, fumarate, acetate,
lactate & glycerol.
(c) Costin Stoica