Phylum Proteobacteria, Class Gammaproteobacteria, Order Vibrionales, Family Vibrionaceae, Genus Photobacterium,
Photobacterium halotolerans Rivas, Garcia-Fraile, Mateos, Martinez-Molina and Velasquez, 2006.
Gram-negative, rod-shaped, 1.7 x 0.7μm. Motile by two polar flagella.
Colonies are smooth, white to cream-coloured, opaque, 2-4 mm in diameter (2 days /
28 ºC). Growth temperature 4-35 ºC, optimum 28 ºC.
Grow on TSA supplemented with 1.5% NaCl, TCBS agar (producing blue colonies).
Growth in nutrient broth with 1 to 8% NaCl (1.5% NaCl optimum), NaCl is not
essential for growth; pH range 5-8.5 (optimum pH 7).
Aerobic or facultatively anaerobic, non-luminescent , chemo-organotrophic,
halotolerant and mesophilic.
Isolated from water collected from a subterranean saline lake in Mallorca, Spain (from a depth of 10 cm).
Shows sensitivity to the vibriostatic agent O/129 and is novobiocin-positive.
- R. Rivas,P.Garcia-Fraile,P.F. Mateos,E. Martinez-Molina and E. Velasquez, 2006. Photobacterium halotolerans sp. nov. , isolated
from Lake Martel in Spain. IJSEM 56, 1067-1071.
Positive results for catalase, oxidase, amylases, beta-galactosidase, alkaline
phosphatase, leucine arylamidase, acid phosphatase, beta-glucosaminidase, hydrolysis of gelatin, reduction of nitrate to nitrite,
fermentation of glucose, mannitol, sucrose & L-arabinose, and uses as sole carbon sources N-acetylglucosamine, citrate, adipate,
glucose, malate, maltose, mannitol, phenylacetate, sucrose, fructose & trehalose.
Negative results for Voges-Proskauer reaction, caseinase, esterase, esterase/lipase, arginine dihydrolase, indole production,
ornithine decarboxylase, lysine decarboxylase, urease, aesculin hydrolysis, lipase, valine arylamidase, cystine arylamidase, trypsin,
chymotrypsin, production of H2S. Does not use as carbon sources amygdalin, caproate, D-arabinose, L-xylose, L-rhamnose,
galactose, sorbose, D-mannose, melibiose, cellobiose, lactose, melezitose, raffinose, turanose, lyxose, tagatose, D-fucose,
L-fucose, methyl alpha-D-xyloside, methyl alpha-D-mannoside, methyl alpha-D-glucoside, arbutin, salicin, inulin, adonitol, inositol,
sorbitol, dulcitol, erythritol, xylitol, arabinitol, glycerol, 2-ketogluconate & 5-ketogluconate.
(c) Costin Stoica