Phylum Proteobacteria, Class Gammaproteobacteria, Order Vibrionales, Family Vibrionaceae, Genus Photobacterium,
Photobacterium frigidiphilum Seo, Bae, Lee and Kim 2005.
Only one strain was tested (the type strain, SL13T ).
Gram-negative rods, 0.8-1.5 x 2.3-3.8 μm, motile by means of a polar flagellum.
Colonies are smooth, cream-coloured, opaque, convex and 2-3 mm in diameter (after
3 days/10 ºC). Grows on Marine broth 2216 Difco containing 1% hexadecane
(incubation at 10 ºC / 7 days on orbital shaker, 120 rpm) and Marine agar 2216E
Difco supplemented with 1 % tributyrin (incubation at 10 ºC / 7 days). Is unable to
grow in the absence of Na+. Require MgCl2 and CaCl2 for growth. Grow in the
presence of 1-4% NaCl (1.5 % NaCl optimum).
Grows between 6-20 ºC, optimum temperature 14 ºC. No growth at 4, 30, 35 or 42 ºC.
Facultative anaerobe, not luminescent, psychrophilic, pH range 5.0-8.5, optimum 6.0.
Isolated from deep-sea sediment samples (1500 m depth) in Papua New Guinea. Susceptible to vibriostatic agent O/129.
- H. J. Seo, S.S.Bae, J.H. Lee and S.J. Kim, 2005. Photobacterium frigidiphilum sp. nov., a psychrophilic, lipolytic bacterium isolated
from deep-sea sediments of Edison Seamount. IJSEM 55, 1661-1666.
Positive results for catalase, oxidase, arginine dihydrolase, beta-galactosidase, beta-glucosidase, protease, lipase, reduction of
nitrate to nitrite, indole production & acid production from glucose.
Can utilize as carbon source: dextrin, glycogen, N-acetyl-D-glucosamine, D-fructose, maltose, D-mannitol, D-mannose, sucrose,
D-trehalose, DL-lactic acid, L-alanine, glycyl L -glutamic acid , L-serine, inosine, uridine, glucose 6-phosphate & myo-inositol.
(c) Costin Stoica