Phylum Proteobacteria, Class Gammaproteobacteria, Order Vibrionales, Family Vibrionaceae, Genus Vibrio, Vibrio fluvialis Lee,
Shread, Furniss and Bryant 1981.
V. fluvialis biogroup I (did not produce gas during fermentation, isolated from cases of human diarrhea and from environment);
V. fluvialis biogroup II (produce gas during fermentation, isolated from the environment but not from humans with diarrhea), later
named V. furnissii.
Historical synonyms: “marine aeromonads”, “Group F” Furniss et al. 1977, “Group EF6” Huq et al. 1980.
Gram-negative, straight or curved rods, variable motility. Sometimes may produce
lateral flagella on solid media. Not swarming on complex media.
Produce yellow colonies on TCBS agar (ferment sucrose). Beta-hemolytic on T-soy
agar with 5% sheep blood. Can grow in nutrient broth with: 1% NaCl, 6% NaCl, 8%
NaCl & 10% NaCl. No growth in 0% or 12% NaCl. Grow at 30 -40 ºC. No growth at 4 ºC.
Isolated from natural waters (estuaries) and animals that lives there; occasional from human clinical samples. Sensible to O/129
vibriostatic agent 10 µg.
Causes sporadic human diarrhea and occasional outbreaks. Gastroenteritis associated with V. fluvialis is described as “cholera-like”
About 20% of isolates give a positive rabbit ileal loop test for enterotoxin.
Some studies have provided additional evidence for enterotoxin or for “enterotoxin-like” molecules.
- J.J. Farmer, M. Janda, 2004. Family I. Vibrionaceae. In: Bergey’s Manual of Systematic Bacteriology, Second edition,Vol two, part
B, George M. Garrity (Editor-in-Chief), pp. 491-546.
- J. G.Holt et al., 1994. Group 5 Facultatively anaerobic Gram-negative rods. Subgroup 2 Family Vibrionaceae. In: Begey’s Manual of
Determinative Bacteriology, 9-th edition, Williams & Wilkins. pp. 190-194.
- Judith A. Johnson, 2006. Vibrio. In: Topley & Wilson’s Microbiology and Microbial Infections, 10 edition, Vol. 2, Bacteriology,
Edward Arnold Ltd., 1507-1523.
Positive for methyl red (1% NaCl), citrate utilization (Simmons), arginine (1% NaCl),
nitrate reduction to nitrite, oxidase, lipase, gelatin hydrolysis (1% NaCl, 22 ºC), acid
production from D-glucose, L-arabinose, D-galactose, maltose, D-mannitol,
D-mannose, sucrose & trehalose.
Negative for indole production (Heart Infusion Broth, 1% NaCl), Voges-Proskauer (1% NaCl), H2S on TSI, urea hydrolysis, esculin
hydrolysis, phenylalanine deaminase, ornithine (1% NaCl), gas production from D-glucose, acid production from dulcitol, glycerol,
myo-inositol, lactose, melibiose, raffinose, L-rhamnose, salicin, D-sorbitol & D-xylose.
Variable for D-arabitol, cellobiose & ONPG test.
(c) Costin Stoica