Vibrio aestuarianus
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Gammaproteobacteria, Order Vibrionales, Family Vibrionaceae, Genus Vibrio,Vibrio aestuarianus  
Tison and Seidler 1983.
On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within the
V. aestuarianus spp.
group,
V. aestuarianus subsp. aestuarianus and V. aestuarianus subsp. francensis.
Synonym: Vibrio aestuarii  Tison and Seidler1983.
Gram-negative, straight or curved rods, 0.5 x 1.5-2.0 µm, motile by a single polar
flagellum.
Can grow in nutrient broth with: 1% & 6% NaCl. No growth in 0%,  8%, 10% & 12%
NaCl. Optimal growth at  20-25 ºC. No growth at 4 ºC or 42 ºC.
Facultative anaerobe. Grows without swarming on Trypticase
Soy Blood Agar,
Thiosulfate Citrate Bile-salts Sucrose
Agar, and MacConkey Agar (above media need
to contain 0.5% NaCl). No growth is observed in media lacking NaCl. Beta-hemolytic
on sheep blood agar.
Isolated from estuary water, shellfish, crabs & oysters of the coast of Oregon, USA. Sensible to O/129 vibriostatic agent (10 & 150 µg).
V. aestuarianus subsp. francensis  is a possible oysters pathogen. Eleven strains were isolated from the hemolymph of live and
moribund oysters
(Crassostrea gigas) in France.
  1. J.J. Farmer, M. Janda, 2004. Family I. Vibrionaceae. In:  Bergey’s Manual of Systematic Bacteriology, Second edition,Vol two, part
    B, George M. Garrity (Editor-in-Chief), pp. 491-546.
  2. Tison, David L., Seidler, Ramon J. Vibrio aestuarianus: a New Species from Estuarine Waters and Shellfish. Int J Syst Bacteriol
    1983 33: 699-702.
  3. Garnier M., Labreuche Y., Nicolas J.-L.  Molecular and phenotypic characterization of Vibrio aestuarianus subsp. francensis
    subsp. nov., a pathogen of the oyster Crassostrea gigas (2008) Systematic and Applied Microbiology, 31 (5), pp. 358-365.  
The characters that differentiate subsp. francensis from subsp. aestuarianus are the growth failure at 35 ºC and  utilization failure of
L-serine, alpha-cyclodextrin, D-mannitol, alpha-glycyl-L-aspartic acid, L-threonine and glucose-1-phosphate.

Positive results for nitrate reduction to nitrite, oxidase, indole (Heart Infusion Broth, 1% NaCl), citrate, arginine (1% NaCl), gelatin
hydrolysis (1% NaCl, 22 ºC), acid
production from: D-glucose, glycerol cellobiose, D-galactose, maltose, D-mannitol, D-mannose,
sucrose & trehalose.

Negative results for Voges-Proskauer test (1% NaCl), H2S, ornithine (1% NaCl), gas from D-glucose, acid production from: D-adonitol,
L-arabinose, dulcitol, myo-inositol, melibiose, raffinose, L-rhamnose, salicin, D-sorbitol & D-xylose.

Lysine (1%NaCl), acid from D-arabitol & lactose are variable.
(c) Costin Stoica
Antibiogram
Encyclopedia
Culture media
Biochemical tests
Stainings
Images
Movies
Articles
Identification
Software
R E G N U M
PROKARYOTAE
Previous page
Back