Poly-beta-hydroxybutyrate inclusions
DESCRIPTION
Procedure for the examination of bacterial capacity to accumulate poly-beta-hydroxybutyrate inclusions. Used mainly for
Pseudomonas and Pseudomonas-like organisms.
PROCEDURE
Cultures are grown in a medium with high carbon-nitrogen ratio.
Sudan  black solution is prepared as 0.3% solution in 70% ethanol, shaken and
allowed  to stand overnight.

Make a smear from the culture, dry and heat-fix it then flood it with Sudan black
solution and wait 15 minutes.

Drain off excess stain, dry the slide, then rinse in water and dry again.
Counterstain with safranin, rinse and dry. Examine with oil immersion microscope.
RESULTS
Poly-beta-hydroxybutyrate inclusions are stained blue-grey to black.  Result may vary
depending on strain tested: Acidovorax or Burkholderia strains give weaker reaction
than Ralstonia.
REFERENCES:
1. Pseudomonas Pathogens of Stone Fruits and Nuts: Classical and Molecular Phytobacteriology. COST873 ESR Training Course
Handbook and Presentations. 6th-9th April 2010, Belgrade,Serbia.
ABIS  online
ADVANCES BACTERIAL
IDENTIFICATION
SOFTWARE
(c) Costin Stoica
Antibiogram
Encyclopedia
Culture media
Biochemical tests
Stainings
Images
Movies
Articles
Identification
Software
R E G N U M
PROKARYOTAE
Previous page
Back