Methylene Blue Stainig
DESCRIPTION
Stainig method that cannot differentiate between bacteria.
REAGENTS
Loffler methylene blue solution: methylene blue crystals 1 g, phenol crystals melted,
96% alcohol 10 ml, distilled water 100 ml.

PROCEDURE
Dry and heat fix the slide, then cover it with methylene blue solution
.
Wait 2 to 5 minutes then rinse in distilled water and dry the slide.

RESULTS
Vegetative forms of bacteria  appear blue colored.
NOTES:
Using old, oxidized Loffler methylene blue solution (stored over few months) capsule
will be pink-colored and the rest of the cell will be blue.

REFERENCES
1. H. Raducanescu, V. Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
2. Dumitru Buiuc, Carmen Panzaru: Coloratii, coloranti si reactivi pentru microscopie, micrometrie. In Dumitru Buiuc, Marian Negut:
Tratat de microbiologie clinica ed. III, 2009, 1173-1215.
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