Positive results for oxidase, nitrate is reduced to nitrite (nitrite is not reduced), tyrosine
hydrolysis, arginine dihydrolase, assimilation of: caprate, malate, citrate, glycerol, D-
glucose, D-fructose, gluconate,Tween 80, methyl pyruvate, acetic acid, cis-aconitic acid,
citric acid, formic acid, beta-hydroxybutyric acid, alpha-ketoglutaric acid, propionic, quinic acid, succinic acid, bromosuccinic acid,
L-alaninamide, D-alanine, L-aspartic acid, L-glutamic acid, L-histidine, hydroxy-L-proline, L-leucine, L-ornithine, L-proline,
L-pyroglutamic acid, D-serine, L-serine, DL-carnitine, gamma-aminobutyric acid, urocanic acid & putrescine.
Negative results for urease, DNase, Tween 80, gelatin hydrolysis, aesculin hydrolysis, starch hydrolysis, lecithinase, levan
production, indole, production, beta-galactosidase, D-glucose fermentation, assimilation/utilization of: adipate, phenylacetate,
erythritol, D-arabinose, L-arabinose, L-xylose, adonitol, methyl beta-xyloside, L-sorbose, L-rhamnose, dulcitol, inositol, D-sorbitol,
methyl alpha-D-mannoside, methyl alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin, D-cellobiose, D-lactose,
D-melibiose, D-sucrose, inulin, D-melezitose, D-raffinose, starch, glycogen, xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose,
D-fucose, L-fucose, L-arabitol, 2-ketogluconate, 5-ketogluconate, alpha-cyclodextrin, dextrin, adonitol, D-galactose, gentiobiose,
myo-inositol, lactulose, maltose, D-mannitol, D-mannose, D-melibiose, methyl beta-D-glucoside, D-psicose, D-raffinose,
D-trehalose, D-galactonic acid lactone, D-glucosaminic acid, itaconic acid, sebacic acid, glucuronamide, L-alanyl glycine, glycyl
L-aspartic acid, glycyl L-glutamic acid, L-phenylalanine, thymidine, phenylethylamine, 2,3-butanediol, DL-alpha-glycerol phosphate,
alpha-D-glucose 1-phosphate & D-glucose 6-phosphate.
(most reactions were determined by API 20NE, API 50 CH & Biolog systems)
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas vranovensis Tvrzova et al. 2006.
Gram-negative, nonspore-forming rods, 1-1.5 x 2-4 μm, occurring singly or in pairs.
Motile by polar flagella.
Colonies on nutrient agar are circular, smooth, nonpigmented and 2-3 mm in
diameter (after 24 hours of cultivation). No fluorescein (King B medium) and
pyocyanine (King A medium) production. Growth occurs at 4-35 ºC, with optimum
growth at 28-35 ºC. Growth occurs in up to 5% NaCl.
Isolated from soil.
- Tvrzova L., Schumann P., Sproer C., Sedlacek I., Pacova Z., Sedo O., Zdrahal Z., Steffen M. & Lang E.: Pseudomonas moraviensis
sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds & emended description of
Pseudomonas asplenii. Int. J. Syst. Evol. Microbiol., 2006, 56, 2657-2663.
(c) Costin Stoica