Oxidize the following substrates: acetic acid, N-acetylglucosamine, alaninamide, alanine, L-alanyl-glycine, gamma-aminobutyric acid,
2-aminoethanol, L-arabinose, D-arabitol, L-asparagine, L-aspartic acid, cis-aconitic acid, bromosuccinic acid, DL-carnitine, citric acid,
formic acid, D-fructose, D-galactonic acid lactone, D-galactose, D-galacturonic acid, alpha-D-glucose, D-gluconic acid, D-glucosaminic
acid, D-glucuronic acid, L-glutamic acid, glycerol, L-histidine, beta-hydroxybutyric acid, gamma-hydroxybutyric acid, p-hydroxyphenyl
acetic acid, hydroxy-L-proline, inosine, meso-inositol, alpha-ketoglutaric acid, DL-lactic acid, malonic acid, D-mannitol, D-mannose,
methylpyruvate, monomethylsuccinate, L-ornithine, L-proline, propionic acid, L-pyroglutamic acid, quinic acid, D-saccharic acid, sebacic
acid, L-serine, D-sorbitol, succinic acid, sucrose, D-trehalose, L-threonine, Tween 40, Tween 80, uridine and urocanic acid (results
obtained with BIOLOG GN microplates).
Oxidation of 2,3-butanediol,
DL-alpha-glycerol phosphate, glycyl L-glutamic acid, alpha-hydroxybutyric acid, alpha-ketobutyric acid,
alphaketovaleric acid, L-leucine, maltose, psicose and turanose is variable.
Oxidation of the other organic substrates included in BIOLOG GN microplates is negative.

Assimilation of N-acetyl-d-glucosamine, cis-aconitate, trans-aconitate, DL-alanine, DL-alpha-amino-n-butyrate,
DL-alpha-amino-n-valerate, L-arabinose, D-arabitol, L-aspartate, betaine, caprate, caprylate, citrate, ethanolamine, beta-D-fructose,
fumarate, D-galactose, D-galacturonate, D-gluconate, D-glucosamine, alpha-D-glucose, D-glucuronate, L-glutamate, glutarate,
DL-glycerate, glycerol, 2-keto-D-gluconate, 2-oxoglutarate, DL-beta-hydroxybutyrate, p-hydroxybenzoate, DL-lactate, D-lyxose, L-malate,
D-mannose, D-mannitol, mucate, myo-inositol, L-proline, propionate, protocatechuate, quinate, D-ribose, D-saccharate, L-serine,
D-sorbitol, succinate, sucrose, D-trehalose, trigonelline, L-tyrosine & D-xylose is positive (results obtained with Biotype 100 strips).
Assimilation of D(-)malate, malonate, putrescine & D(-)tartrate is variable.
Other substrates of  Biotype 100 strips are not assimilated.
P. brassicacearum, P. thivervalensis strains does not oxidize succinamic acid and does not assimilate benzoate.
Pseudomonas thivervalensis
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas thivervalensis  
Achouak et al. 2000, sp. nov.
Gram-negative motile rods, 1.0-1.5 μm in length, about 0.5 μm diameter.
Forms mucoid colonies with regular margins when grown on TSA and produces
brown-orange difusible pigment. Strictly aerobic, optimum growth temperature 30 ºC.
Media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar, King's B medium.
Isolated from Brassica napus & Arabidopsis thaliana rhizoplane.
Brassica napus & Arabidopsis thaliana root-associated bacteria.
  1. Achouak W., Sutra L., Heulin T., Meyer J.M., Fromin N., Degraeve S., Christen R. & Gardan L.: Pseudomonas brassicacearum sp.
    nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis
    thaliana. Int. J. Syst. Evol. Microbiol., 2000, 50, 9-18.
  2. Ivanova E.P., Christen R., Bizet  C., Clermont D., Motreff L., Bouchier C., Zhukova N.V., Crawford R.J. & Kiprianova E.A.:
    Pseudomonas brassicacearum subsp. neoaurantiaca subsp. nov., orange-pigmented bacteria isolated from soil and the
    rhizosphere of agricultural plants. Int. J. Syst. Evol. Microbiol., 2009, 59, 2476-2481.
Oxidase-positive, arginine dihydrolase-positive, produces levan, not pectinolytic.
Reduction of nitrate to nitrite is variable.
Hydrolyses gelatin, but not aesculin.
Acid is produced from sucrose and sorbitol but not from erythritol & mannitol.
(c) Costin Stoica
Culture media
Biochemical tests
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