Oxidize the following substrates: acetic acid, N-acetylglucosamine, alaninamide, alanine, L-alanyl-glycine, gamma-aminobutyric acid,
2-aminoethanol, L-arabinose, D-arabitol, L-asparagine, L-aspartic acid, cis-aconitic acid, bromosuccinic acid, DL-carnitine, citric acid,
formic acid, D-fructose, D-galactonic acid lactone, D-galactose, D-galacturonic acid, alpha-D-glucose, D-gluconic acid, D-glucosaminic
acid, D-glucuronic acid, L-glutamic acid, glycerol, L-histidine, beta-hydroxybutyric acid, gamma-hydroxybutyric acid, p-hydroxyphenyl
acetic acid, hydroxy-L-proline, inosine, meso-inositol, alpha-ketoglutaric acid, DL-lactic acid, malonic acid, D-mannitol, D-mannose,
methylpyruvate, monomethylsuccinate, L-ornithine, L-proline, propionic acid, L-pyroglutamic acid, quinic acid, D-saccharic acid, sebacic
acid, L-serine, D-sorbitol, succinic acid, sucrose, D-trehalose, L-threonine, Tween 40, Tween 80, uridine and urocanic acid (results
obtained with BIOLOG GN microplates).
Oxidation of 2,3-butanediol, dl-a-glycerol phosphate, glycyl L-glutamic acid, alpha-hydroxybutyric acid, alpha-ketobutyric acid,
alphaketovaleric acid, L-leucine, maltose, psicose and turanose is variable.
Oxidation of the other organic substrates included in BIOLOG GN microplates is negative.

Assimilation of N-acetyl-d-glucosamine, cis-aconitate, trans-aconitate, DL-alanine, DL-alpha-amino-n-butyrate,
DL-alpha-amino-n-valerate, L-arabinose, D-arabitol, L-aspartate, betaine, caprate, caprylate, citrate, ethanolamine, beta-D-fructose,
fumarate, D-galactose, D-galacturonate, D-gluconate, D-glucosamine, alpha-D-glucose, D-glucuronate, L-glutamate, glutarate,
DL-glycerate, glycerol, 2-keto-D-gluconate, 2-oxoglutarate, DL-beta-hydroxybutyrate, p-hydroxybenzoate, DL-lactate, D-lyxose, L-malate,
D-mannose, D-mannitol, mucate, myo-inositol, L-proline, propionate, protocatechuate, quinate, D-ribose, D-saccharate, L-serine,
D-sorbitol, succinate, sucrose, D-trehalose, trigonelline, L-tyrosine & D-xylose is positive (results obtained with Biotype 100 strips).
Assimilation of D(-)malate, malonate, putrescine & D(-)tartrate is variable.
Other substrates of  Biotype 100 strips are not assimilated.
P. brassicacearum, P. thivervalensis strains do not oxidize succinamic acid & do not assimilate benzoate.
Pseudomonas thivervalensis
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas thivervalensis  
Achouak et al. 2000, sp. nov.
Gram-negative motile rods, 1.0-1.5 μm in length, about 0.5 μm diameter.
Forms mucoid colonies with regular margins when grown on TSA and produces
brown-orange difusible pigment. Strictly aerobic, optimum growth temperature 30 ºC.
Media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar, King's B medium.
Isolated from Brassica napus & Arabidopsis thaliana rhizoplane.
Brassica napus & Arabidopsis thaliana root-associated bacteria.
  1. Achouak W., Sutra L., Heulin T., Meyer J.M., Fromin N., Degraeve S., Christen R. & Gardan L.: Pseudomonas brassicacearum sp.
    nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis
    thaliana. Int. J. Syst. Evol. Microbiol., 2000, 50, 9-18.
  2. Ivanova E.P., Christen R., Bizet  C., Clermont D., Motreff L., Bouchier C., Zhukova N.V., Crawford R.J. & Kiprianova E.A.:
    Pseudomonas brassicacearum subsp. neoaurantiaca subsp. nov., orange-pigmented bacteria isolated from soil and the
    rhizosphere of agricultural plants. Int. J. Syst. Evol. Microbiol., 2009, 59, 2476-2481.
Oxidase-positive, arginine dihydrolase-positive, produces levan, not pectinolytic.
Reduction of nitrate to nitrite is variable.
Hydrolyses gelatin, but not aesculin.
Acid is produced from sucrose and sorbitol but not from erythritol & mannitol.
(c) Costin Stoica
Culture media
Biochemical tests
Previous page