Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Burkholderiaceae, Genus Ralstonia, Ralstonia
syzygii (Roberts et al. 1990) Vaneechoutte et al. 2004.
Historical synonym: Pseudomonas syzygii Roberts et al. 1990.
Gram-negative, nonsporulating, noncapsulated, nonmotile, straight rods with
rounded ends (0.5-0.6 x 1.0-2.5 μm that occur singly, in pairs or in short chains.
Aerobic. Optimum temperature for growth is 28 ºC. No growth occurs at 4 or 37 ºC.
Growth is poor (colonies of <1 mm after 7 days) or absent on many common media.
Good growth (colonies up to 5 mm after 7-12 days) occurs on iron salts-containing
media: buffered charcoal yeast extract agar, Periwinkle wilt medium and
iron-supplemented casamino acids medium. Grow at pH 6.0-7.5. Does not grow in
the presence of 1% NaCl; variable growth in 0.5% NaCl.
Isolated from xylem tissues of the clove tree (Syzygium aromaticum) and other Syzygium spp., and from insect vectors (Hindola spp.)
in Indonesia. Resistant to colistin.
Pathogenic to the clove tree (Syzygium aromaticum).
- Vaneechoutte, Mario, Kampfer, Peter, De Baere, Thierry, Falsen, Enevold, Verschraegen, Gerda Wautersia gen. nov., a novel
genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia
[Pseudomonas] syzygii (Roberts et al. 1990) comb. nov. Int J Syst Evol Microbiol 2004 54: 317-327.
Positive results for oxidase, catalase, alkaline and acid phosphatases, esterase, esterase lipase, leucine arylamidase, urease,
naphthol-AS-BI-phosphohydrolase, utilization of fumarate, DL-malate, pyruvate, succinate, L-aspartate, L-glutamate, L-tyrosine,
D-glucose & sucrose.
Negative results for levan production, arginine dihydrolase , lecithinase, aesculin hydrolysis, starch hydrolysis, trypsin, chymotrypsin,
alpha- or beta-galactosidases, beta-glucuronidase, alpha- or beta-glucosidases, N-acetyl-b-glucosamidase, alpha-mannosidase or
alpha-fucosidase, utilization of beta-alanine, L-arginine, L-cysteine, glycine, L-leucine, L-lysine, L-methionine, L-phenylalanine,
DL-serine, L-threonine, L-trytophan, L-valine, L-arabinose, D-cellobiose, D-galactose, lactose, maltose, D-mannose, D-raffinose,
D-rhamnose, D-ribose, D-xylose, acetate, DL-lactate, D-tartrate, m-erythritol, glycerol, mannitol & sorbitol.
Variable results for tyrosinase activity, nitrate reduction, utilization of L-asparagine, L-histidine, fructose, citrate & 2-oxoglutarate.
(c) Costin Stoica