Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas psychrotolerans Hauser et al. 2004.
Gram-negative, nonspore-forming rods, 0.5-0.7 x 1.5-1.8 μm. Motile.
Colonies are yellow, irregular, leathery, dry, wrinkled, form within 48 hours, with a
diameter of 1-5 mm. After 3-5 days, colonies can be up to 8 mm in diameter. No
fluorescent pigment producing.
Growth occurs under aerobic and microaerobic, but not anaerobic, conditions.
Media: PYE agar, TSA, R2A and MacConkey agar, PYE agar supplemented with 1-5%
(w/v) NaCl. Temperature 4-37 ºC. No growth occurs at 42 ºC.
Isolated from water under a dog’s cage and from a strip of metal under the treatment table in an animal clinic in Austria.
- Hauser, Elke, Kampfer, Peter, Busse, Hans-Jurgen. Pseudomonas psychrotolerans sp. nov. Int J Syst Evol Microbiol 2004 54:
Positive results for catalase, acid production from: glucose, L-arabinose, D-xylose,
melibiose, D-mannose, D-mannitol, inositol, trehalose & D-arabitol.
Can assimilate: D-glucose, putrescine, fumarate, glutarate, DL-3-hydroxybutyrate,
4-aminobutyrate, DL-lactate, L-malate, oxoglutarate, pyruvate, 4-hydroxybenzoate, L-alanine, L-proline, L-serine, D-trehalose,
D-fructose, L-arabinose, D-galactose, D-mannose, D-mannitol, D-xylose, i-inositol, D-sorbitol, gluconate, D-maltose, D-ribose,
cis-aconitate, trans-aconitate, L-aspartate, citrate, acetate, propionate, mesaconate, & itaconate.
Negative results for oxidase, hydrolysis of aesculin, acid production from: lactose, sucrose, dulcitol, salicin, adonitol, raffinose,
cellobiose, methyl D-glucoside & erythritol.
No assimilation of beta-alanine, L-ornithine, phenylacetate, L-phenylalanine, assimilation of N-acetyl-D-galactosamine,
N-acetyl-D-glucosamine, p-arbutin, D-cellobiose, alpha-D-melibiose, sucrose, salicin, adonitol, maltitol, adipate, azelate,
L-tryptophan, 3-hydroxybenzoate & suberate.
Variable results for assimilation of: L-histidine, L-leucine & L-rhamnose, acid production from: sorbitol, rhamnose & maltose.
(c) Costin Stoica