Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas proteolytica Reddy et al. 2004.
Gram negative rods, motile by a polar flagellum.
On solid medium: circular, convex, smooth and translucent colonies with a diameter
of 1–2 mm. Aerobic, psychrophilic - growth temperature 4-30 ºC (optimum 22 ºC),
48h. Can tolerate 3% NaCl (w/v). Optimum pH = 7.
Trypticase Soy Agar ± 5% sheep blood, Antarctic Bacterial Medium (0.5% peptone,
0.2% yeast extract & 1.5% agar at pH 6.9).
Isolated from cyanobacterial mat samples (water) that were collected from Antarctica.
- Reddy GSN; Matsumoto GI; Schumann P; Stackebrandt E; Shivaji S.: Psychrophilic pseudomonads from Antarctica:
Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov. Int J Syst Evol
Positive results for catalase, oxidase, lipase, gelatinase, nitrate reduction to nitrite, acid
production from: D-fructose, D-galactose, D-glucose, D-mannose & D-mannitol.
Utilizes as sole carbon sources: acetate, adonitol, citrate, mesoerythritol, D-fructose,
D-galactose, D-glucose, glycerol, meso-inositol, lactic acid, D-mannose, D-mannitol,
pyruvate, D-ribose, sorbitol, trehalose, L-alanine, L-arginine, L-glutamic acid, L-glutamine, L-lysine, L-serine, L-methionine, L-
phenylalanine, L-tyrosine & L-tryptophan.
Negative results for phosphatase, urease, beta-galactosidase, arginine dihydrolase, lysine decarboxylase, indole production, methyl
red, Voges–Proskauer test, aesculin hydrolysis, starch hydrolysis, cellulose hydrolysis, acid production from L-arabinose, lactose, L-
rhamnose, sucrose & D-xylose.
Not utilized: D-cellobiose, cellulose, dextran, dulcitol, fumaric acid, inulin, lactose, D-maltose, D-melibiose, melezitose, D-raffinose, L-
rhamnose, D-sorbose, sucrose, succinic acid, thioglycollate, D-xylose, L-aspartic acid, L-aspargine, L-cysteine, L-glycine, L-histidine,
L-leucine, L-isoleucine, L-proline, L-threonine & L-valine.
(c) Costin Stoica