Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas pertucinogena Kawai and Yabuuchi 1975.
Synonyms: Bordetella pertusis rough variants (strains ATCC 190 and ATCC 6627).
Gram negative rods, noncapsulated, 0.4 x 1.1 μm, motile by a single polar flagellum.
No accumulation of poly-beta-hydroxybutyrate.
Colonies on Bordet-Gengou medium after 24 h of incubation at 37 ºC are grayish, 1
mm in diameter, and mimicked the colonies of rough phase IV strains of B . pertussis.
Colonies of the two strains were less than 1 mm in diameter, semitranslucent, entire,
smooth, and glistening on brain heart infusion agar, Trypticase soy agar, heart
infusion agar & nutrient agar. No pigment production, no hemolysis on rabbit blood
agar. Produce a slight, homogeneous turbidity in brain heart infusion broth after 24
hours at 30 ºC.
Aerobic. Grow at 41 ºC. Grow in BHI containing 3% NaCl, Bordet-Genou medium. No
growth on LD agar, SS agar, Pseudosel agar & NAC agar.
The source of isolation of the strains ATCC 190 and ATCC 6627 is unknown but, their former identification as strains of B . pertussis
suggests that they were isolated from the human respiratory tract.
Resistant to novobiocin. Both strains produce a bacteriocin ('pertucin') that inhibits the growth of B . pertussis.
Unknown. Experimental infection: dermal necrosis in guinea pigs appeared at 24 h after injection of ultrasonically disrupted cell
suspensions of ATCC 190 and ATCC 6627 only at a concentration of 10" cells/ml or higher.
- Kawai, Yoko, Yabuuchi, Eiko. Pseudomonas pertucinogena sp.nov., an Organism Previously Misidentified as Bordetella pertussis.
Int J Syst Bacteriol 1975 25: 317-323.
- George M. Garrity, Julia A. Bell & Timothy Lilburn: Order IX Pseudomonadales Orla-Jensen 1921In: Bergey’s Manual of
Systematic Bacteriology, Second edition,Vol two, part B, George M. Garrity (Editor-in-Chief), pp. 323-442.
Positive results for oxidase, catalase, H2S (lead acetate paper) & phenylalanine deaminase.
Utilize as carbon source: L-alpha- alanine, pyruvate, succinate, oxaloacetate & p-hydroxybutyrate.
Negative results for indole, methyl red, acetylmethylcarbinol, Simmons citrate, Christensen citrate, nitrate reduction to nitrite, lysine
decarboxylase, ninhydrin, L-lysine, L-ornithine decarboxylase, L-arginine dihydrolase (Moller), acylamidase, urease, phosphatase,
deoxyribonuclease, hydrolysis of gelatin, starch & Tween 80, acid production from: inositol, mannitol, sorbitol, lactose, maltose,
cellobiose, melibiose, sucrose, trehalose, raffinose, melezitose, inulin & salicin.
Not utilized: D-arabinose, L-arabinose, D-ribose, xylose, rhamnose, glucose, fructose, galactose, mannose, lactose, maltose,
cellobiose, sucrose, trehalose, raffnose, salicin, inulin, ethanol, adonitol, inositol, mannitol, sorbitol, dulcitol, glycerol, L-glycine,
L-leucine, L-isoleucine, L-serine, L-threonine, L-valine, L-aspartic acid, L-glutamic acid, L-proline, L-tryptophane, L-phenylalanine,
L-tyrosine, L-histidine, L-arginine, L-lysine, L-ornithine, L-methionine, alpha-, beta- & gamma- amino-n-butyric acid.
(c) Costin Stoica