Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas pertucinogena Kawai and Yabuuchi 1975.
Synonyms: Bordetella pertusis rough variants (strains ATCC 190 and ATCC 6627).
Gram-negative rods, noncapsulated, 0.4 x 1.1 μm, motile by a single polar flagellum.
No accumulation of poly-beta-hydroxybutyrate.
Colonies on Bordet-Gengou medium after 24 h of incubation at 37 ºC are grayish, 1
mm in diameter, and mimicked the colonies of rough phase IV strains of B. pertussis.
Colonies of the two strains were less than 1 mm in diameter, entire, smooth,
semitranslucent, and glistening on brain heart infusion agar, Trypticase soy agar,
heart infusion agar & nutrient agar. No pigment production, no hemolysis on rabbit
blood agar. Produce a slight, homogeneous turbidity in brain heart infusion broth after
24 hours at 30 ºC. Aerobic. Grow at 41 ºC. Grows in BHI containing 3% NaCl,
Bordet-Genou medium. No growth on LD agar, SS agar, Pseudosel agar & NAC agar.
The source of isolation of the strains ATCC 190 and ATCC 6627 is unknown but, their former identification as strains of Bordetella
pertussis suggests that they were isolated from the human respiratory tract.
Resistant to novobiocin. Both strains produce a bacteriocin ('pertucin') that inhibits the growth of Bordetella pertussis.
Unknown. Experimental infection: dermal necrosis in guinea pigs appeared at 24 h after injection of ultrasonically disrupted cell
suspensions of ATCC 190 and ATCC 6627 only at a concentration of 10" cells/ml or higher.
- Kawai, Yoko, Yabuuchi, Eiko. Pseudomonas pertucinogena sp.nov., an Organism Previously Misidentified as Bordetella pertussis.
Int J Syst Bacteriol 1975 25: 317-323.
- George M. Garrity, Julia A. Bell & Timothy Lilburn: Order IX Pseudomonadales Orla-Jensen 1921 In: Bergey’s Manual of
Systematic Bacteriology, Second edition,Vol two, part B, George M. Garrity (Editor-in-Chief), 2005, pp. 323-442.
Positive results for oxidase, catalase, H2S (lead acetate paper) & phenylalanine deaminase.
Utilize as carbon source: L-alpha- alanine, pyruvate, succinate, oxaloacetate & p-hydroxybutyrate.
Negative results for indole, methyl red, acetylmethylcarbinol, Simmons citrate, Christensen citrate, nitrate reduction to nitrite, lysine
decarboxylase, ninhydrin, L-lysine, L-ornithine decarboxylase, L-arginine dihydrolase (Moller), acylamidase, urease, phosphatase,
deoxyribonuclease, hydrolysis of gelatin, starch & Tween 80, acid production from: inositol, mannitol, sorbitol, lactose, maltose,
cellobiose, melibiose, sucrose, trehalose, raffinose, melezitose, inulin & salicin.
Not utilized: D-arabinose, L-arabinose, D-ribose, xylose, rhamnose, glucose, fructose, galactose, mannose, lactose, maltose,
cellobiose, sucrose, trehalose, raffnose, salicin, inulin, ethanol, adonitol, inositol, mannitol, sorbitol, dulcitol, glycerol, L-glycine,
L-leucine, L-isoleucine, L-serine, L-threonine, L-valine, L-aspartic acid, L-glutamic acid, L-proline, L-tryptophane, L-phenylalanine,
L-tyrosine, L-histidine, L-arginine, L-lysine, L-ornithine, L-methionine, alpha-, beta- & gamma- amino-n-butyric acid.
(c) Costin Stoica