Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas panipatensis Gupta et al. 2008.
Gram negative, nonsporulating rods, 1.0–1.2 x 2.8–3.5 μm. Motile by a single polar
Colonies are smooth, circular and muddy-white, growing up to 1.5 mm diameter on
Luria Bertani agar after 72 hours incubation at 30 ºC.
Aerobic. No growth at 4 ºC; grows at 41 ºC, optimum temperature 28–37 ºC and pH 5–
9, but growth does not occur in the presence of 3% NaCl. Growth is observed on
MacConkey agar, Mueller–Hinton agar, Luria Bertani medium.
Isolated from oil-contaminated refinery soil in India.
- Gupta, Sanjay Kumar, Kumari, Rekha, Prakash, Om, Lal, Rup. Pseudomonas panipatensis sp. nov., isolated from an oil-
contaminated site. Int J Syst Evol Microbiol 2008 58: 1339-1345.
Positive for oxidase, catalase, DNase, nitrate reductase, urease, acid production
from D-glucose & maltose.
Can utilize as carbon source: D-glucose, D-fructose, D-galactose, D-arabinose,
trehalose, heptane, sorbitol, acetate, L-proline, o-cresol, allantoin, pyruvate & citrate.
Negative results for lecithinase, esculin hydrolysis, gelatin hydrolysis, indole production, methyl red, Voges–Proskauer, amylase, acid
production from: adonitol, mannitol, ribose, D-fructose, D-galactose, D-arabinose, sucrose, xylose, raffinose & lactose.
Egg yolk, D-xylose, lactose, raffinose, pyridine, cystine, D-mannitol,D- ribose, D-galactose, L-arabinose, toluene, glycerol, L-serin,
sorbose, inositol, pyrocatechal, aniline, para-nitrophenol & alpha-naphtol are not utilized.
(c) Costin Stoica