Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas pachastrellae Romanenko et al. 2005
Gram-negative rods, encapsulated, 1.4-1.6 x 0.4-0.5 μm, motile by means of a single
Colonies on are circular, smooth, non-pigmented, whitish and transparent, 2-3 mm in
diameter, on TSA and MA. Fluorescent pigments and pyocyanin are not produced on
King B and King A medium, respectively. No hemolysis.
Aerobic. Growth is observed in 0-10% (w/v) NaCl (optimally at 5% NaCl) and at
temperatures of 7-41 ºC; no growth occurs at 4 or 42 ºC; optimal temperature 28 ºC.
Grows on media: King A & B agar, Trypticase Soy Agar, Marine agar / broth.
Isolated from a marine sponge collected from the Philippine Sea at a water depth of 750 m.
- Romanenko, Lyudmila A., Uchino, Masataka, Falsen, Enevold, Frolova, Galina M., Zhukova, Natalia V., Mikhailov, Valery V.
Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 2005 55: 919-924.
Positive results for catalase, hydrolysis of Tween 40, esterase C4, esterase lipase C8, phosphoamidase, leucine arylamidase,
oxidase, utilization of: citrate, DL-lactate & L-tyrosine.
Negative results for denitrification, urease, lysine decarboxylase, ornithine decarboxylase, arginine dihydrolase, acetoin production, H2S
production, indole production, aesculin hydrolysis, gelatin hydrolysis, starch hydrolysis, DNA hydrolysis, alkaline phosphatase, lipase
C14, valine arylamidase, cystine arylamidase, alpha-chymotrypsin, trypsin, acid phosphatase, alpha- and beta-galactosidase, beta-
glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, alpha- mannosidase, alpha-fucosidase, utilization of: L-
arabinose, phenylacetate, DL-norleucine, N-acetyl-glucosamine, D-glucose, maltose, xylose, fructose, D-mannose, sucrose, trehalose,
glycerol, D-mannitol, gluconate, malate, L-valine & L-lysine.
Acid is not produced from D-glucose, D-xylose, rhamnose, galactose, maltose, lactose & mannitol.
Production of acid from glycerol is a variable character.
(c) Costin Stoica