Pseudomonas pachastrellae
Taxonomy
Morphology
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Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas pachastrellae  Romanenko et al. 2005
Gram-negative rods, encapsulated, 1.4-1.6 x 0.4-0.5 μm, motile by means of a single  
flagellum.
Colonies on nutrient agar are circular, smooth, non-pigmented, whitish and
transparent, 2-3 mm in diameter, on TSA and MA. Fluorescent pigments and
pyocyanin are not produced on King B and King A medium, respectively. No
hemolysis.
Aerobic. Growth is observed in 0-10% (w/v) NaCl and at temperatures of 7-41 ºC; no
growth occurs at 4 or 42 ºC; optimal temperature 28 ºC. Grows on media: King A & B
agar, Trypticase Soy Agar, Marine agar / broth.
Isolated from a marine sponge collected from the Philippine Sea at a water depth of 750 m.
Unknown.
  1. Romanenko, Lyudmila A., Uchino, Masataka, Falsen, Enevold, Frolova, Galina M., Zhukova, Natalia V., Mikhailov, Valery V.
    Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 2005 55: 919-924.
Positive results for oxidase, catalase,  hydrolysis of Tween 40, esterase C4, esterase lipase C8, phosphoamidase, leucine
arylamidase, utilization of: citrate, DL-lactate & L-tyrosine.

Negative results for denitrification, urease, lysine decarboxylase, ornithine decarboxylase, arginine dihydrolase, acetoin production, H
2S
production, indole production, aesculin hydrolysis, gelatin hydrolysis, starch hydrolysis, DNA hydrolysis, alkaline phosphatase, lipase
C14, valine arylamidase, cystine arylamidase, alpha-chymotrypsin, trypsin, acid phosphatase, alpha-galactosidase,
beta-galactosidase, beta-glucuronidase, alpha-glucosidase, beta-glucosidase, N-acetyl-beta-glucosaminidase, alpha- mannosidase,
alpha-fucosidase, utilization of: L-arabinose, phenylacetate, DL-norleucine & N-acetyl-glucosamine, D-glucose, maltose, xylose,
fructose, D-mannose, sucrose, trehalose, glycerol, D-mannitol, gluconate, malate, L-valine & L-lysine.
Acid is not produced from D-glucose, D-xylose, rhamnose, galactose, maltose, lactose & mannitol.

Production of acid from glycerol is a variable character.
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