Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas nitroreducens Iizuka and Komagata 1964.
Synonym: Pseudomonas multiresinivorans Mohn et al. 1999.
Gram-negative rods, 0.4 to 0.6 by 1.4 to 1.8 μm, occurring singly or in pairs. Motile by
Colonies on nutrient agar are circular, smooth or slightly rough, entire, raised,
glistening, pale yellowish brown, opalescent, butyrous. On nutrient agar slant:
moderate growth, filiform, glistening, pale brownish yellow. Fluorescence may be
present in early stages of isolation. Optimum temperature 25-30 ºC. No growth at 4 ºC
or 37 ºC. Aerobic. Able to grow in the presence of 3-5% NaCl. Growth media: Nutrient
Agar / Broth, Glutamate Agar / Broth, MacConkey agar.
Isolated from an oil brine in Japan.
- Iizuka (H.) and Komagata (K.): Microbiological studies on petroleum and natural gas. I. Determination of hydrocarbon-utilizing
bacteria. Journal of General and Applied Microbiology, 1964, 10, 207-221.
- Lang, Elke, Griese, Barbara, Sproer, Cathrin, Schumann, Peter, Steffen, Maike, Verbarg, Susanne. Characterization of
'Pseudomonas azelaica' DSM 9128, leading to emended descriptions of Pseudomonas citronellolis Seubert 1960 (Approved Lists
1980) and Pseudomonas nitroreducens Iizuka and Komagata 1964 (Approved Lists 1980), including Pseudomonas
multiresinivorans as its later heterotypic synonym. Int J Syst Evol Microbiol 2007 57: 878-882.
- Gupta, Sanjay Kumar, Kumari, Rekha, Prakash, Om, Lal, Rup. Pseudomonas panipatensis sp. nov., isolated from an oil-
contaminated site. Int J Syst Evol Microbiol 2008 58: 1339-1345.
Positive results for nitrate reduction to nitrite and gas, cytochrome oxidase, catalase, hydrolysis of Tween 80, hydrolysis of
hypoxanthine, acid production from: glucose, fructose & glycerol, utilization of adipate, citrate, glycerol, L-histidine, hydroxy-L-proline,
N-acetylgalactosamine, trans-aconitate, mesaconate, fumarate, glutarate, pyruvate & 4-hydroxybenzoate.
Can utilize kerosene and crude oil as the sole source of carbon and energy.
Negative results for Voges-Proskauer reaction, ornithine decarboxylase, indole production from tryptophan, H2S production,
hydrolysis of gelatin, hydrolysis of aesculin, hydrolysis of starch, beta-galactosidase, lecithinase, acid production from: adonitol,
dulcitol, mannitol, ribose, xylose, galactose & arabinose.
No utilization of: L-arabiose, mannitol, sorbitol, xylose, malonate, 2-ketogluconate, 5-keto-gluconate, ethanol, salicylate, gentisate,
anthranilate, p-amino-benzoate, o-aminobenzoate, benzylformate, D-galactose, acetate, hippurate & propionate.
Variable results for urease, utilization of: L-serine, mucate & 2-butanol.
(c) Costin Stoica