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Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas marincola  
Romanenko et al. 2008.
Gram-negative, motile, rods, 1.8-2.5 x 0.4-0.6 µm.
Colonies are smooth, nonpigmented, whitish and translucent, 3-4 mm in diameter.
Brown diffusible pigments produced on peptone-enriched media. No production of
pyocyanin on King A medium or fluorescent pigments on King B medium.
Strictly aerobic, optimal growth temperature 25-28 ºC. Can grow at 4 but not over 38
ºC. Growth is observed in 0-8% (w/v) NaCl. Optimum pH 6.5-8.5.
Media: Tryptic Soya Agar, Tryptic Soya Broth, Marine Agar 2216 & Marine Broth 2216.
Isolated from an ophiuran (brittle-star, an organism of the class Ophiuroidea resembling the starfish but having long slender arms)
specimen collected from the Fiji Sea.
Unknown.
  1. Romanenko, Lyudmila A., Uchino, Masataka, Tebo, Bradley M., Tanaka, Naoto, Frolova, Galina M., Mikhailov, Valery V.
    Pseudomonas marincola sp. nov., isolated from marine environments.Int J Syst Evol Microbiol 2008 58: 706-710.
Description is based on API 20NE, API ID32 GN and API 50 CH results.

Positive results for catalase, oxidase, H
2S production (slow), arginine dihydrolase & nitrate reduction. Can assimilate: caprate,
malate, citrate, itaconic acid, sodium malonate, sodium acetate, lactic acid, propionic acid, capric acid, trisodium citrate, L-alanine,
L-proline, 3-hydroxybutyric acid & 4-hydroxybenzoic acid.

Negative results for indole production, urease, gelatinase, starch hydrolysis, aesculin hydrolysis, ONPG & acid production from
D-glucose, arabinose, mannose, rhamnose, galactose, maltose, fructose, lactose, D-xylose, mannitol & glycerol.
Negative for the assimilation of  D-glucose, L-arabinose, D-mannose, D-mannitol, maltose, sucrose, trehalose, D-galactose,
D-xylose, D-ribose, glycerol, 2-ketogluconate, 5-ketogluconate, gluconate, N-acetylglucosamine, adipate, erythritol, D-arabinose,
L-xylose, D-adonitol, methyl beta-D-xyloside, L-sorbose, L-rhamnose, dulcitol, inositol, D-sorbitol, methyl alpha - D-mannoside,
methyl alpha-D-glucoside, amygdalin, arbutin, aesculin, salicin, cellobiose, D-lactose, melibiose, inulin, melezitose, raffinose, starch,
glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose, D-fucose, L-fucose, L-arabitol, suberic acid, 3-hydroxybenzoic acid,
L-serine, valeric acid & L-histidine.

Variable results for the hydrolysis of Tweens 80 and 40  & assimilation of phenylacetate.
Pseudomonas marincola
(c) Costin Stoica
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