Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas marincola Romanenko et al. 2008.
Gram-negative, motile, rods, 1.8-2.5 x 0.4-0.6 µm.
Colonies are smooth, nonpigmented, whitish and translucent, 3-4 mm in diameter.
Brown diffusible pigments produced on peptone-enriched media. No production of
pyocyanin on King A medium or fluorescent pigments on King B medium.
Strictly aerobic, optimal growth temperature 25-28 ºC. Can grow at 4 but not over 38
ºC. Growth is observed in 0-8% (w/v) NaCl. Optimum pH 6.5-8.5.
Media: Tryptic Soya Agar, Tryptic Soya Broth, Marine Agar 2216 & Marine Broth 2216.
Isolated from an ophiuran (brittle-star, an organism of the class Ophiuroidea resembling the starfish but having long slender arms)
specimen collected from the Fiji Sea.
- Romanenko, Lyudmila A., Uchino, Masataka, Tebo, Bradley M., Tanaka, Naoto, Frolova, Galina M., Mikhailov, Valery V.
Pseudomonas marincola sp. nov., isolated from marine environments.Int J Syst Evol Microbiol 2008 58: 706-710.
Description is based on API 20NE, API ID32 GN and API 50 CH results.
Positive results for catalase, oxidase, H2S production (slow), arginine dihydrolase & nitrate reduction. Can assimilate: caprate,
malate, citrate, itaconic acid, sodium malonate, sodium acetate, lactic acid, propionic acid, capric acid, trisodium citrate, L-alanine,
L-proline, 3-hydroxybutyric acid & 4-hydroxybenzoic acid.
Negative results for indole production, urease, gelatinase, starch hydrolysis, aesculin hydrolysis, ONPG & acid production from
D-glucose, arabinose, mannose, rhamnose, galactose, maltose, fructose, lactose, D-xylose, mannitol & glycerol.
Negative for the assimilation of D-glucose, L-arabinose, D-mannose, D-mannitol, maltose, sucrose, trehalose, D-galactose,
D-xylose, D-ribose, glycerol, 2-ketogluconate, 5-ketogluconate, gluconate, N-acetylglucosamine, adipate, erythritol, D-arabinose,
L-xylose, D-adonitol, methyl beta-D-xyloside, L-sorbose, L-rhamnose, dulcitol, inositol, D-sorbitol, methyl alpha - D-mannoside,
methyl alpha-D-glucoside, amygdalin, arbutin, aesculin, salicin, cellobiose, D-lactose, melibiose, inulin, melezitose, raffinose, starch,
glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose, D-fucose, L-fucose, L-arabitol, suberic acid, 3-hydroxybenzoic acid,
L-serine, valeric acid & L-histidine.
Variable results for the hydrolysis of Tweens 80 and 40 & assimilation of phenylacetate.
(c) Costin Stoica