Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas libanensis Dabboussi et al. 1999.
Colonies on nutrient agar are smooth, circular and non-pigmented. Non-haemolytic
on blood agar. Growth temperature ranges between 4 and 36 ºC; optimal at 30 ºC.
Growth occurs in the presence of 3% NaCl but not in 7 % NaCl. It is able to form levan
Gram-negative, nonspore-forming rods, motile by one polar flagellum. Cells produce
a fluorescent pigment on King B medium, but not phenazine pigments on King A
medium. Poly-beta-hydroxybutyrate is not accumulated as a carbon reserve material.
Isolated from Lebanese spring waters.
- Dabboussi, F., Hamze, M., Elomari, M., Verhille, S., Baida, N., Izard, D., Leclerc, H. Pseudomonas libanensis sp. nov., a new
specie isolated from Lebanese spring waters. Int J Syst Bacteriol 1999 49: 1091-1101.
Positive results for alkaline phosphatase, arginine dihydrolase, nitrates reduction,
catalase, oxidase, lecithinase, acid phosphatase, esterase, leucinearylamidase,
hydrolysis of L-tyrosine, urease, utilization of citrate, malonate, D- & L-arabitol, myo-inositol, xylitol, D-mannitol, D-sorbitol, adonitol,
erythritol, D-mannose, D-galactose, L-arabinose, D-xylose, ribose, D-glucosamine, ethanolamine, D-saccharate, mucate,
2-ketogluconate, protocatechuate, beta-hydroxybenzoate, 3-hydroxybutyrate, D-glucuronate, D-galacturonate, itaconate & quinate.
Negative results for lipase, elastase, tetrathionate reductase, lysine decarboxylase, ornithine decarboxylase, alpha-chymotrypsin,
alpha-galactosidase, beta-galactosidase, beta-glucuronidase, alpha-glucosidase, alpha-mannosidase, alpha-fucosidase, esculin,
Voges-Proskauer reaction, utilization of maltitol, sucrose, alpha-L-fucose, D-turanose, alpha-D-melibiose, D-raffinose, maltotriose,
maltose, D-cellobiose, beta-gentiobiose, palatinose, D-tagatose, alpha-lactose, lactulose, 1-O-methyl-beta-galactopyranoside,
1-O-methyl alpha-galactopyranoside, 3-O-methyl-gamma-glycopyranose, 5-keto-D-gluconate, tricarballylate, phenylacetate, gentisate,
L-histidine, m-hydroxybenzoate, 5-aminovalerate, D-tartrate, benzoate, aesculin, trigonelline, histamine, tryptamine, L-tryptophan &
inulin. The strains do not denitrify.
Variable results for the utilization of: D-trehalose & meso-tartrate.
(c) Costin Stoica