Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas japonica Pungrasmi et al. 2008.
Gram-negative, motile by means of polar multitrichous flagella, nonspore-forming,
straight rods, 2.0–3.5 x 1.3–1.7 μm.
Colonies are circular, flat, opaque, creamy white to pale yellow with slightly spreading
edges on nutrient agar. Pyocyanin and fluorescent pigments are not produced.
Aerobic, can grow in nutrient broth at 30 and 37 ºC, not at 4 or 42 ºC. Fluorescent
pigment production was tested on King A & King B medium. On CFMM/APs agar get
visible colonies in 3 days at 30 ºC.
Isolated from an activated sludge seed collected from a sewage treatment plant in Japan.
- Wiboonluk Pungrasmi, Haeng-Seog Lee, Akira Yokota & Akinori Ohta. Pseudomonas japonica sp. nov., a novel species that
assimilates straight chain alkylphenols. The Journal of General and Applied Microbiology Vol. 54 (2008) , No. 1 pp.61-69.
Positive results for oxidase & catalase.
Can utilize the following substrates as sole carbon sources for growth: glucose,
caprate, malate, citrate, phenyl-acetate, D-xylose, D-fucose, alpha-cyclodextrin,
glycogen, Tween 40, Tween 80, L-arabinose, D-fructose, L-fucose, maltose, beta-methyl-D-glucoside, D-psicose, L-rhamnose,
turanose, xylitol, methyl pyruvate, monomethylsuccinate, acetic acid, cis-aconitic acid, citric acid, formic acid, D-glucuronic acid,
beta-hydroxybutyric acid, p-hydroxyphenyl acetic acid, itaconic acid, alpha-ketobutyric acid, alpha-keto glutaric acid, alpha-keto valeric
acid, D-lactic acid, L-lactic acid, malonic acid, propionic acid, quinic acid, D-saccharic acid, succinic acid, bromosuccinic acid,
succinamic acid, glucuronamide, alanine amide, D-alanine, L-alanine, L-alanyl-glycine, L-asparagine, L-aspartic acid, L-glutamic
acid, glycyl-L-aspartic acid, hydroxyl-L-proline, L-leucine, L-ornithine, L-phenylalanine, L-proline, L-pyroglutamic acid, D-serine,
L-serine, D-carnitine, L-carnitine, g-aminobutyric acid, urocanic acid, inosine, uridine, phenyl ethylamine, putrescine, 2-amino ethanol,
glycerol, glucose-1-phosphate & glucose-6-phosphate.
The following enzymes are produced: protease, esterase (C4), esterase lipase (C8), lipase (C14), leucine arylamidase,
valine arylamidase, acid phosphatase and naphthol-AS-BI-phosphohydrolase.
Can utilize a wide range of para-substituted straight chain alkylphenols, namely from 1 up to 9 carbon atoms as a carbon
source for growth.
Negative results for nitrate reduction, denitrification, arginine dihydrolase production, alkaline phosphatase, trypsin, assimilation of
arabinose, mannose, mannitol, L-arabinose, ribose, 2-keto-gluconate, utilization of dextrin, D-galactose, D-galacturonic acid,
D-glucosamic acid, alpha-hydroxybutyric acid, gamma-hydroxybutyric acid, 2,3-butanediol, D,L-alpha-glycerol phosphate.
(c) Costin Stoica