Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas guineae Bozal et al. 2007.
Gram-negative rods, 0.4-0.5 x 1.5-2 µm, non-spore-forming, motile by means of polar
After 72 h incubation at 15 ºC on TSA, colonies are 1.5–2.0 mm in diameter, smooth
and round with irregular edges. Do not produce fluorescent pigment on King’s B
Aerobic, psychrotolerant. Growth occurs at temperatures between -4 and 30 °C, but
not at 37 ºC. NaCl is tolerated at concentrations up to 4% (w/v).
Isolated from antarctic soil (South Shetland Islands).
Positive results for oxidase, catalase, alkaline phosphatase, esterase, esterase lipase,
lipase, valine arylamidase, cystine arylamidase, acid phosphatase, leucine
arylamidase, naphthol-AS-BI-phosphohydrolase, lecithin hydrolysis, utilization of
caprate, malate &citrate.
Negative results for nitrate reduction, casein hydrolysis, starch hydrolysis, Tween 80, DNA hydrolysis, trypsin, chymotrypsin,
alpha-galactosidase, beta-galactosidase, beta-glucuronidase, beta-glucosidase, beta-glucosidase, N-acetyl-beta-glucosaminidase,
alpha-mannosidase, alpha-fucosidase, indole production, acidification of glucose, arginine dihydrolase, urease, aesculin hydrolysis,
gelatin hydrolysis, assimilation of glycerol, L-arabinose, D-ribose, D-glucose, D-fructose, D-mannose, D-mannitol,
N-acetyl-glucosamine, D-maltose, D-sucrose, D-trehalose, L- and D-arabitol, gluconate, adipate, phenylacetate, erythritol,
D-arabinose, L-xylose, D-adonitol, methyl beta-D-xyloside, L-sorbose, L-rhamnose, dulcitol, inositol, D-sorbitol, methyl
alpha-D-mannoside, methyl alpha-D-glucoside, amygdalin, arbutin, aesculin, salicin, D-cellobiose, D-lactose, D-melibiose, inulin,
D-melezitose, D-raffinose, starch, glycogen, xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose, D- and L-fucose, 2- or 5-
ketogluconate & D-galactose.
- Bozal, Nuria, Montes, M. Jesus, Mercade, Elena. Pseudomonas guineae sp. nov., a novel psychrotolerant bacterium from an
Antarctic environment. Int J Syst Evol Microbiol 2007 57: 2609-2612.
(c) Costin Stoica