Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas graminis Behrendt et al. 1999.
Gram-negative, aerobic, nonspore-forming, straight rods (0.5-1.0 by 3.5-5.0 μm),
motile by one polar flagellum (motile at 15 & 20 ºC; nonmotile at 30 ºC).
Colonies are yellow, glistening, moderately convex and round with entire edges.
Beta-hemolysis of sheep blood is negative. Does not produce fluorescent pigments
on King medium B. Does not produce levan from sucrose. Growth temperature 15-30
ºC. Can grow at 4 ºC but not at 42 ºC. Media: King medium B, Mac Conkey agar.
Isolated from the phyllosphere of grasses in Germany.
- Behrendt U., Ulrich A., Schumann P., Erler W., Burghardt J. & Seyfarth W.: A taxonomic study of bacteria isolated from grasses: a
proposed new species Pseudomonas graminis sp. nov. Int. J. Syst. Bacteriol., 1999, 49, 297-308.
- George M. Garrity, Julia A. Bell & Timothy Lilburn: Order IX Pseudomonadales Orla-Jensen 1921 In: Bergey’s Manual of
Systematic Bacteriology, Second edition,Vol two, part B, George M. Garrity (Editor-in-Chief), 2005, pp. 323-442.
Positive for catalase production, hydrolysis of Tween 40, 80 & aesculin, utilization of
adonitol, L-arabinose, D-arabitol, D-fructose, L-fucose, D-galactose, alpha-D-glucose,
myo-inositol, D-mannitol, D-mannose, D-sorbitol, methylpyruvate, monomethyl
succinate, cis-aconitic acid, citric acid, formic acid, D-galactonic acid, D-galacturonic acid, D-gluconic acid, D-glucosaminic acid,
D-glucuronic acid, P-hydroxybutyric acid, itaconic acid, alpha-ketoglutaric acid, D,L-lactic acid, malonic acid, D-saccharic acid, succinic
acid, bromosuccinic acid, succinamic acid, glucuronamide, D-alanine, L-alanine, L-alanylglycine, L-asparagine, L-aspartic acid,
L-glutamic acid, L-histidine, L-proline, L-pyroglutamic acid, gamma-aminobutyric acid, urocanic acid, inosine, uridine,
2-aminoethanol & glycerol.
Negative for oxidase, glucose oxidation & fermentation, starch hydrolysis, arginine dihydrolase, lysine decarboxylase, ornithine
decarboxylase, tryptophan desaminase, DNase, urease, beta-galactosidase, Voges-Proskauer, indole production, hydrogen
sulphide production, reduction of nitrate to nitrite & denitrification.
Utilization of N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, cellobiose, i-erythritol, gentiobiose, alpha-D-lactose, lactulose,
maltose, D-melibiose, P-methyl-D glucoside, D-raffinose, L-rhamnose, sucrose, D-trehalose, p-hydroxyphenylacetic acid, quinic acid,
sebacic acid, glycyl-L-aspartic acid, L-phenylalanine, thymidine, phenylethylamine, putrescine, 2,3- butanediol, glucose-1-phosphate,
glucose-6-phosphate, alpha-cyclodextrin, dextrin & glycogen is negative.
Variable results for the utilization of D-psicose, turanose, xylitol, acetic acid, alpha-hydroxybutyric acid, alpha-ketobutyric acid,
alpha-ketovaleric acid, propionic acid, alaninamide, glycyl-L-glutamic acid, hydroxy-L-proline, L-leucine, L-ornithine, D,L-serine,
L-threonine, D,L-carnitine & D,L-alpha-glycerolphosphate.
(c) Costin Stoica