Catalase and Kovacs oxidase tests are positive. Lipolysis of Tween 80 is positive Peptonization and reduction of litmus milk without
coagulation are positive. Gelatin & starch are hydrolyzed. Arginine dihydrolase and ammonia are produced. Acid is produced from
glucose, arabinose, rhamnose, mannitol mannose & trehalose.
L-arabinose, acetate, beta-alanine, citrate, malonate, succinate, urate, trehalose, L-valine & L-lysine are utilized

Denitrification is negative; nitrate is not reduced. Methyl red,  Voges-Proskauer test are negative. Esculin and arbutin are not.
hydrolyzed. Cytochrome oxidase, phenylalanine deaminase, urease, 2-ketogluconate, H
2S,and indole are not produced.
Beta-glucosidase & tyrosinase negative. Levan is not produced from sucrose.
Acid is not produced from adonitol, dextrin, dulcitol, erythritol, inositol, lactose, maltose, inulin, raffinose, salicin, sorbitol, sucrose,
xylose or alpha-methylglucoside.
Tartrate, hippurate, 2-ketogluconate, inositol, sorbitol, adonitol & polygalacturonic acid are not utilized.
Pseudomonas fuscovaginae
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas fuscovaginae (ex Tanii et al. 1976) Miyajima et al. 1983.
Gram-negative , nonspore-forming, rod-shaped cells with round ends, 0.5 to 0.8 x 2.0
to 3.5 μm. The cells occur singly or in pairs and are motile by means of one to four
polar flagella.
White to light brown, smooth, glistening, raised, translucent, circular, butyrous
colonies 3 to 5 mm in diameter is produced after 4 to 5 days at 28 ºC on nutrient agar.
Produce a green fluorescent, diffusible pigment on King's medium B. No slime is
produced on nutrient agar containing 5% sucrose.
Aerobic. The optimal growth temperature is approximately 28 ºC. No growth occurs at
37 ºC.
No growth occurs in nutrient broth supplemented with 5% NaCl.
Isolated from diseased leaf sheaths of 0ryza sativa in Japan.
Pathogenic for the Gramineae. Is the causative agent of sheath brown rot of rice.
Pathogenicity was demonstrated on
Oryza sativa, Hordeum vulgare, Triticum aestivum, Avena sativa, Zea mays, Lolium perenne,
Bromus marginatus, Phleum pratense & Phalaris arundinacea.
Produce a hypersensitivity reaction in tobacco plants.
  1. Miyajima K., Tanii A. & Akita T.: Pseudomonas fuscovaginae sp. nov., nom. rev. Int. J. Syst. Bacteriol., 1983, 33, 656-657.
  2. P. Rott: Identification of Pseudomonas fuscovaginae with Biochemical, Serological, and Pathogenicity Tests. Plant disease /
    August 1991, 843-846.
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