|Brevundimonas (Pseudomonas) diminuta|
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas diminuta Leifson and Hugh 1954. Moved to Phylum Proteobacteria, Class Alphaproteobacteria, Order Caulobacterales,
Family Caulobacteraceae, Genus Brevundimonas, Brevundimonas diminuta (Leifson and Hugh 1954) Segers et al. 1994 (type
species of the genus).
(Synonym: Bacterium parvulum)
Gram-negative, 0.5 x 1.0-4.0 μm, motile rods by one polar flagellum.
Smooth, glistening, opaque, circular, convex with entire edge colonies. No pigment
Strictly aerobic, optimal temperature 30 ºC. Can grow at 41 ºC, not at 4 ºC.
Pantothenate, biotin, cystine and cyanocobalamin are required as growth factors.
Media: Nutritive agar, Trypticase Soy Agar, Mueller-Hinton agar, TSI, Mac Conkey.
Isolated from water & clinical specimens.
Unknown. Is commonly used as a test organism for the efficiency of water filters due to the small size of the bacterium.
- Bergey’s Manual of Determinative Bacteriology, 9th ed., 1994.
- Leifson E. & Hugh R.: A new type of polar monotrichous flagellation. Journal of General Microbiology, 1954, 10, 68-70.
- Kaltenbach C. M., Moss C. W. & Weaver R. E.: Cultural and biochemical characteristics and fatty acid composition of
Pseudomonas diminuta and Pseudomonas vesiculare. J Clin Microbiol. 1975 April; 1(4): 339–344.
Accumulate poli-beta-hydroxybutyrate as intracellular carbon reserve, especially in
nitrogen-deficient media. Denitrification negative, nitrite rarely produced from nitrate.
Tween 80 starch, gelatin, egg yolk reaction negative.
Positive results for oxidase, catalase positive utilization of acetate, butyrate, L-leucine, L-serine, L-histidine, DL-norvaline, and
L-rhamnose, mannitol, L-arabinose, sorbitol, sucrose, D-cellobiose, starch, DL-lactate, D-xylose, adonitol, D-mannose, D-glucose,
D-galactose, arbutin, salicin, maltose, trehalose, propionate, amygdalin, isobutyrate n -caproate, adipate, pimelate, suberate, azelate,
sebacate, glycine and aconitate are not utilized
Esculin is not hydrolyzed. Negative for valine arylamidase, alpha- & beta-glucosidase activities. Indole is not formed.
Utilization of valerate, succinate, fumarate, L-malate, 2-ketoglutarate, D-alpha-alanine, L-alanine, L-isoleucine, L-valine, L-norleucine,
DL-norvaline, L-threonine, L-tyrosine & L-arginine is variable.
(c) Costin Stoica