Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas congelans Behrendt et al. 2003.
Gram-negative, non-sporeforming rods, motile by one polar flagellum, 2.5-2.9 µm
wide and about 0.8 µm long.
Aerobic, optimal growth temperature 21 ºC. Media: Trypticase Soy Agar, Nutrient agar,
King’s agar A & B. Colonies on King A and B media are white-yellowish, smooth with
regular margins and produce a pigment with light yellow-green fluorescence on UV
light. Nonhemolytic on sheep blood agar.
First isolated from the phyllosphere of grasses in Germany. Presents ice nucleation activity.
Possible plant pathogen.
- Behrendt U., Ulrich A. & Schumann P.: Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas
trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov. Int. J. Syst. Evol. Microbiol., 2003, 53, 1461-
- Sarah E. Burr, Stefanie Gobeli, Peter Kuhnert, Elinor Goldschmidt-Clermont, and Joachim Frey: Pseudomonas chlororaphis
subsp. piscium subsp. nov., isolated from freshwater fish. Int J Syst Evol Microbiol December 2010 60:2753-2757.
Positive results for gelatinase, casein hydrolysis, catalase, aesculin hydrolysis & aerobic acid formation from glucose.
Can utilize: acetate, citrate, gluconate, caprate, DL-lactate, malonate, L-alanine, L-arabinose, D-arabitol, m-inositol, D-mannitol,
sucrose, D-galacturonic acid, D-glucosaminic acid, D-glucuronic acid, L-asparagine, L-pyroglutamic acid, L-threonine, D-trehalose,
glycerol, DL-lactic acid, 2-aminoethanol, i-erythritol, alpha-D-glucose, glycyl, L-glutamic acid, DL-alpha-glycerol phosphate, alpha-
hydroxybutyric acid, beta-hydroxybutyric acid, formic acid, L-histidine, monomethyl succinate, L-proline, D-psicose, quinic acid, D-
sorbitol, L- and D-serine, succinamic acid, & Tween 40.
Negative for arginine dihydrolase, DNase, beta-galactosidase, indole production, formation of H2S from sodium thiosulphate,
reduction of nitrate to nitrite, denitrification, oxidase, hydrolysis of starch, formation of levan from sucrose & urease.
No utilization of: propionate, 2- and 5-ketogluconate, maltose, adipate, itaconate, D-tartrate, N-acetyl-D-glucosamine, DL-carnitine, D-
cellobiose, glucose 6-phosphate, glycyl L-aspartic acid, gamma-hydroxybutyric acid, hydroxy-L-proline, glycogen, p-
hydroxyphenylacetic acid, alpha-ketovaleric acid, L-ornithine, putrescine & L-rhamnose.
(c) Costin Stoica