Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas brenneri Baida et al. 2002, emend. van den Beld et al. 2016.
Gram negative rods. Motile by means of a single polar flagellum. Poly-beta-
hydroxybutyrate is not accumulated.
Grows on MacConkey agar, Salmonella Shigella (SS agar and Cetrimide and in BHI)
with 3 % (w/v) NaCl. Variable growth in BHI with 5 % (w/v) NaCl. No growth in BHI with
7, 9 and 10 % (w/v) NaCl. Grows at 4 ºC and 30 ºC, with weak growth at 37 ºC and no
growth at 41 ºC. Does not grow anaerobically on CSA. No beta-haemolysis. Produces
fluorescent pigment (pyoverdin) on King B medium,
Isolated from French natural mineral waters.
- Van Den Beld, M. J., Reinders, E., Notermans, D. W. and Reubsaet, F. A. 2016. Possible misidentification of species in the
Pseudomonas fluorescens lineage as Burkholderia pseudomallei and Francisella tularensis, and emended descriptions of
Pseudomonas brenneri, Pseudomonas gessardii and Pseudomonas proteolytica. Int. J. Syst. Evol. Microbiol., 66, 3420-3425.
- Baida N., Yazourh A., Singer E. and Izard D.: Pseudomonas brenneri sp. nov., a new species isolated from natural mineral waters.
Res. Microbiol., 2001, 152, 493-502.
Positive results for arginine dihydrolase, caseinase, catalase, esterase lipase (C8), lecithinase, leucine arylamidase, nitrate
reduction, nitrite reduction, oxidase, acid phosphatase & naphtol-AS-BI-phosphohydrolase.
Produce acid from D-glucose, L-rhamnose, D-mannitol, D-mannose, D-fructose & D-galactose.
Can utilize D-glucose, D-mannose, D-mannitol, N-acetyl-glucosamine, gluconate, caprate, adipate, malate & citrate
Negative results for H2S-production from TSI, indole-production, esculin hydrolysis, methyl red, phenylanaline deamination, starch
hydrolysis, lipase (C14), cystine arylamidase, trypsin, alpha-chymotrypsin, alpha-galactosidase, beta-galactosidase, beta-
glucuronidase, alpha-glucosidase, beta-glucosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase, alpha-fucosidase, lysine
decarboxylase, ornithine decarboxylase, methyl red, Voges-Proskauer test, urease & DNase.
Acid is not produced from lactose, D-maltose & D-sucrose.
Does not utilize: L-arabinose, Na-malonate, phenyl acetate & maltose.
Variable results for alkaline phosphatase, gelatin hydrolysis, esterase (C4), valine arylamidase & D-xylose acidification.
(c) Costin Stoica