Oxidize the following substrates: acetic acid, N-acetylglucosamine, alaninamide, alanine, L-alanyl-glycine, gamma-aminobutyric acid,
2-aminoethanol, L-arabinose, D-arabitol, L-asparagine, L-aspartic acid, cis-aconitic acid, bromosuccinic acid, DL-carnitine, citric acid,
formic acid, D-fructose, D-galactonic acid lactone, D-galactose, D-galacturonic acid, alpha-D-glucose, D-gluconic acid,
D-glucosaminic acid, D-glucuronic acid, L-glutamic acid, glycerol, L-histidine, beta-hydroxybutyric acid, gamma-hydroxybutyric acid,
p-hydroxyphenyl acetic acid, hydroxy-L-proline, inosine, meso-inositol, alpha-ketoglutaric acid, DL-lactic acid, malonic acid,
D-mannitol, D-mannose, methylpyruvate, monomethylsuccinate, L-ornithine, L-proline, propionic acid, L-pyroglutamic acid, quinic
acid, D-saccharic acid, sebacic acid, L-serine, D-sorbitol, succinamic acid, succinic acid, sucrose, D-trehalose, L-threonine, Tween
40, Tween 80, uridine and urocanic acid (results obtained with BIOLOG GN microplates).
Oxidation of 2,3-butanediol, DL-alpha-glycerol phosphate, glycyl L-glutamic acid, alpha-hydroxybutyric acid, alpha-ketobutyric acid,
alphaketovaleric acid, L-leucine, maltose, psicose and turanose is variable.
Oxidation of the other organic substrates included in BIOLOG GN microplates is negative.

Assimilation of N-acetyl-D-glucosamine, cis-aconitate, trans-aconitate, DL-alanine, DL-alpha-amino-n-butyrate, DL-alpha-amino-n-
valerate, L-arabinose, D-arabitol, L-aspartate, betaine, caprate, caprylate, citrate, ethanolamine, beta-D-fructose, fumarate,
D-galactose, D-galacturonate, D-gluconate, D-glucosamine, alpha-D-glucose, D-glucuronate, L-glutamate, glutarate, DL-glycerate,
glycerol, 2-keto-D-gluconate, 2-oxoglutarate, DL-beta-hydroxybutyrate, p-hydroxybenzoate, DL-lactate, D-lyxose, L-malate, D-mannose,
D-mannitol, mucate, myo-inositol, L-proline, propionate, protocatechuate, quinate, D-ribose, D-saccharate, L-serine, D-sorbitol,
succinate, sucrose, D-trehalose, trigonelline, L-tyrosine & D-xylose is positive (results obtained with Biotype 100 strips).
Assimilation of benzoate, D(-)malate, malonate, putrescine & D(-)tartrate is variable.
Other substrates of  Biotype 100 strips are not assimilated.
P. brassicacearum, P. thivervalensis strains does not oxidize succinamic acid and does not assimilate benzoate.
Pseudomonas brassicacearum
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas brassicacearum
Achouak et al. 2000. 2 subspecies:
Subsp. brassicacearum  Achouak et al. 2000,
Subsp. neoaurantiaca  Ivanova et al. 2009.
Gram-negative rods, 1.0-1.5 μm x 0.5 μm in diameter.
Forms mucoid colonies with regular margins. Brown-orange diffusible pigment and a
fluorescent pigment are produced. Strictly aerobic, optimum growth temperature is 30
ºC. Media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar, King's B medium.
Isolated from Brassica napus & Arabidopsis thaliana rhizoplane.
Brassica napus root-associated bacteria. Possible pathogenic action on tomato. No hypersensitivity reaction produced on tobacco
  1. Achouak W., Sutra L., Heulin T., Meyer J.M., Fromin N., Degraeve S., Christen R. & Gardan L.: Pseudomonas brassicacearum sp.
    nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis
    thaliana. Int. J. Syst. Evol. Microbiol., 2000, 50, 9-18.
  2. Ivanova E.P., Christen R., Bizet  C., Clermont D., Motreff L., Bouchier C., Zhukova N.V., Crawford R.J. & Kiprianova E.A.:
    Pseudomonas brassicacearum subsp. neoaurantiaca subsp. nov., orange-pigmented bacteria isolated from soil and the
    rhizosphere of agricultural plants. Int. J. Syst. Evol. Microbiol., 2009, 59, 2476-2481.
  3. Sarah E. Burr, Stefanie Gobeli, Peter Kuhnert, Elinor Goldschmidt-Clermont, and Joachim Frey: Pseudomonas chlororaphis
    subsp. piscium subsp. nov., isolated from freshwater fish. Int J Syst Evol Microbiol December 2010 60:2753-2757.
Oxidase-positive, arginine dihydrolase-positive, produces levan, not pectinolytic.
Reduction of nitrate to nitrite is variable.
Hydrolyses gelatin, but not aesculin or urea. No beta-glactosidase activity.
Acid is produced from sucrose and sorbitol but not from erythritol & mannitol.
(c) Costin Stoica
Culture media
Biochemical tests
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