Phylum Proteobacteria, Class Gammaproteobacteria, Order Pasteurellales, Family Pasteurellaceae, Genus Haemophilus,
Haemophilus felis Inzana, Johnson, Shell, Møller and Killian 1999.
Gram-negative, 0.45-0.55 x 1.5-1.7 µm, coccobacilli or small rods, with occasional
Colonies on chocolate agar incubated in air plus 10% extra CO2 are raised, smooth,
and rounded 0.5-1.0 mm in diameter after 24 h.
Colonies on Brain-heart infusion agar supplemented with NAD are very adherent and
show yellow pigmentation when collected on a loop.
Weak hemolysis after 3 days incubation. CAMP test negative. Require increased
concentration of CO2 in incubation atmosphere for primary isolation but this
requirement is lost upon subcultivation. Require V-factor but not X-factor for growth.
Optimum temperature 35-37 °C. Growth on Mac Conkey agar is negative.
Has been isolated from the nasopharynxes of 6 of 28 apparently normal cats and from the lower respiratory tract of a cat with chronic
obstructive pulmonary disease.
Has been isolated from the lower respiratory tract of a cat with chronic obstructive pulmonary disease.
- Mogens Kilian, 2004. Genus III. Haemophilus. In: Bergey’s Manual of Systematic Bacteriology, Second edition, Vol two, part B,
George M. Garrity (Editor-in-Chief), pp. 883-904.
- Mary P.E. Slack, 2006. Haemophilus. In: Topley & Wilson’s Microbiology and Microbial Infections, 10 edition, Vol. 2, Bacteriology,
Edward Arnold Ltd.
- Inzana T.J., Johnson J.L., Shell L., MØller K., Kilian M., 1992. Isolation and characterization of a newly identified Haemophilus
species from cats: ”Haemophilus felis”. J. Clin. Microbiol., 30, 2108-2112.
Positive results for nitrates reduction, alkaline phosphatase, catalase, H2S production, alpha-glucosidase, acid production from: D(+)
glucose (without gas), mannitol, D(+) galactose, D(+) mannose, lactose, maltose & sucrose (saccharose).
Negative results for oxidase, urease, lysine decarboxylase, ornithine decarboxylase, ONPG, indole production, acid production from: L
(+) arabinose, D(+) xylose, meso-inositol, D(-) sorbitol, D(+) melibiose, trehalose, raffinose, inulin, esculin & salicin.
(c) Costin Stoica