Colonies on Blood agar are small, circular, raised, yellowish, smooth, shining.
Growth is often adherent to the medium. Chromogenesis best seen on Loeffler's
serum medium; usually a bright greenish yellow. Haemolysis is not produced.
Optimum temperature, 37 ºC. Can grow at 22 ºC. Aerobic, facultatively anaerobic.
Taxonomy
Morphology
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Biochemical characters
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Pathogenicity
References
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Neisseriaceae, Genus Neisseria, Neisseria perflava
Bergey et al. 1923.

According to Bergey’s Manual of Systematic Bacteriology, Second edition, 2005,
Neisseria subflava contains the previous species N.
flava
, N. perflava, and N. subflava, resulting 3 biovars: N. subflava biovar flava, N. subflava biovar perflava, N. subflava biovar
subflava.
However, it seems that Bergey's consolidation of the species was not official and in the Approved Lists of Bacterial Names
(Amended) 1989, they are distinct species.
Gram-negative cocci, 0.5-1.0 μm in diameter, occurring singly and in pairs with adjacent sides flattened. Nonmotile. Not capsulated.
Endospores are not produced.
Isolated from the nasopharynx, saliva and sputum. Habitat: Mucous membrane of respiratory tract of man. The most common of the
chromogenic Neisseria. Susceptible to colistin.
Undetermined.
  1. Tone Tonjum, 2005. Order IV. Neisseriales ord. nov. In:  Bergey’s Manual of Systematic Bacteriology, Second edition, Vol two, part C
    The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, George M. Garrity (Editor-in-Chief), pp 774-863.
  2. Euzeby (J.P.): List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int. J. Syst. Bacteriol., 1997,
    47, 590-592. (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net).
  3. Magnus Unemo (Editor-in-Chief), 2013. Laboratory diagnosis of sexually transmitted infections, including human immunodeficiency
    virus, pp 44-45. World Health Organization, ISBN 978 92 4 150584 0.
  4. Daniel C. Stein, 2006. The Neisseria. In: The Prokaryotes Third Edition, Volume 5: Proteobacteria: Alpha and Beta Subclasses,
    Martin Dworkin (Editor-in-Chief), pp 602–647.
  5. Skerman (V.B.D.), McGowan (V.) and Sneath (P.H.A.) (editors): Approved Lists of Bacterial Names. Int. J. Syst. Bacteriol., 1980, 30,
    225-420.
  6. Robert S. Breed, E. G. D. Murray, Nathan R. Smith, 1957. Family VIII. Neisseriaceae Prevot, 1933. In: Bergey’s Manual of
    Determinative Bacteriology, 7th edition, 480-485.
Positive results for carbonic anhydrase, catalase, iodine test (polysaccharide
production), nitrite reduction (with production of gas), oxidase, acid production from:
fructose, glucose, maltose & sucrose.

Negative results for beta-galactosidase (ONPG), DN-ase, gamma-glutamyl
transferase, nitrate reduction, tributyrin hydrolysis, acid production from: lactose &
mannose.
Neisseria perflava
(c) Costin Stoica
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