Colonies grown on an R2A agar plates for 2 days are yellowish and circular. Slightly
thermophilic, growing at 10-55 ºC (optimal growth at 40-50 ºC); no growth occurs at 5
ºC or 60 ºC. The pH range for growth is pH 6.0-10.0 (optimal growth at pH 8.0-9.0).
Growth occurs with 0-3% (w/v) NaCl (optimal growth with 0-1%) and no growth occurs
with 4.0% (w/v) NaCl.
Phylum Proteobacteria, Class Betaproteobacteria, Order Neisseriales, Family Neisseriaceae, Genus Crenobacter, Crenobacter
luteus Dong et al. 2015 - type species of the genus.
Gram-negative short rods, 0.4-0.6 μm wide and 1.2-1.7 μm long. Motile.
Isolated from a sediment sample collected at Hehua hot spring, Tengchong, Yunnan province, south-west China.
- Dong, L., Ming, H., Zhou, E.-M., Yin, Y.-R., Liu, L., Feng, H.-G., Xian, W.-D., Nie, G.-X. and Li, W.-J. 2015. Crenobacter luteus gen.
nov., sp. nov., isolated from a hot spring. Int. J. Syst. Evol. Microbiol., 65, 214-219.
- Euzeby J.P., List of Prokaryotic names with Standing in Nomenclature, Genus Crenobacter, http://www.bacterio.net/crenobacter.
html, accessed 05.2017.
Description is based on API ZYM, API 20 NE, API 50 CH and Biolog test results.
Positive results for alkaline phosphatase, catalase, esterase C4 & C8, esterase lipase, naphthol-AS-BI-phosphohydrolase, indole
production, nitrate reduction, oxidase, acid production from: amygdalin, arbutin, erythritol, fructose, glucose, glycerol, 5-ketogluconate,
D-lyxose, maltose, ribose, sucrose & D-tagatose.
Can utlize acetic acid, bromosuccinic acid, capric acid, dextrin, alpha-D-glucose, D-fructose 6-phospate, D-fructose, D-glucose 6-
phospate, D-galacturonic acid, D-glucuronic acid, D-malic acid, maltose, trehalose, turanose, D-serine, L-arginine, L-aspartic acid,
L-galactonic acid lactone, L-glutamic acid, L-histidine, L-lactic acid, L-malic acid, L-serine, glucuronamide, maltose, methyl pyruvate,
pectin, propionic acid & sucrose.
Negative results for arginine dihydrolase, casein hydrolysis, citrate utilization, cystine arylamidase, esculin hydrolysis, alpha-
fucosidase, N-acetyl-beta-glucosaminidase, lipase C14, gelatin hydrolysis, alpha- or beta-galactosidase, beta-glucuronidase, alpha-
glucosidase, leucine arylamidase, alpha-mannosidase, starch hydrolysis, trypsin, chymotrypsin, Tween 80 hydrolysis, urease &
(c) Costin Stoica