Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Enterococcaceae, Genus Vagococcus, Vagococcus penaei Jaffres et
Gram-positive cocci, 0.5-1 µm in diameter, occuring singly, in pairs or in short chains,
elongated in the direction of the chain. Non-motile. Non-spore-forming.
Colonies are small (up to 1 mm in diameter), smooth and white when grown on BHI
agar at 30 ºC. No haemolysis or pigment on Columbia agar supplemented with 5 %
defibrinated horse blood. Facultatively anaerobic. Lactic acid is produced from
glucose fermentation, but gas is not produced. Grows at 10 ºC but not at 45 ºC. Does
not grow with 6.5 % NaCl.
Isolated from the spoilage microbiota of cooked shrimp (Penaeus vannamei).
- Jaffres (E.), Prevost (H.), Rossero (A.), Joffraud (J.J.) and Dousset (X.): Vagococcus penaei sp. nov., isolated from spoilage
microbiota of cooked shrimp (Penaeus vannamei). Int. J. Syst. Evol. Microbiol., 2010, 60, 2159-2164.
- Killer, J., Svec, P., Sedlaccek, I., Cernohlavkova, J., Benada, O., Hroncova, Z., Havlik, J., Vlkova, E., Rada, V., Kopecny, J. and
Kofronova, O. 2014. Vagococcus entomophilus sp. nov., from the digestive tract of a wasp (Vespula vulgaris). Int. J. Syst. Evol.
Microbiol., 64, 731-737.
Positive results for acid phosphatase, arginine dihydrolase, esterase (C4), esterase lipase (C8), glycyl-tryptophan arylamidase,
naphthol-AS-BI-phosphohydrolase, pyroglutamic acid arylamidase, Voges– Proskauer test, acid production from: maltose,
melezitose, raffinose, D-ribose, sucrose, trehalose & alpha-cyclodextrin.
Negative results for alpha-chymotrypsin, catalase, indole production, alpha-fucosidase, alpha- and beta-galactosidase, alpha- and
beta-glucosidase, beta-glucuronidase, hippurate hydrolysis, alpha- and beta-mannosidase, alkaline phosphatase, lipase (C14),
alanyl-phenylalanyl-proline arylamidase, cystine arylamidase, leucine arylamidase, nitrate reduction, oxidase, valine arylamidase, N-
acetyl-beta-glucosaminidase, trypsin, urease, acid production from: L-arabinose, D-arabitol, lactose, D-mannitol, melibiose, D-
sorbitol, D-tagatose, glycogen, methyl beta-D-glucopyranoside & pullulan.
(c) Costin Stoica