Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Enterococcaceae, Genus Vagococcus, Vagococcus entomophilus
Killer et al. 2014.
Gram-positive, ovoid cocci, 0.7-0.9 x 0.5-1.1 µm, occuring single or grouped in pairs.
Colonies on TSYE agar under aerobic conditions after 48 h incubation at 37 ºC are
generally triangular or disc-shaped with regular edges, white, smooth and reach 0.82-
2.75 mm in diameter. Grows at 10 ºC and 40 ºC. Grows at pH 5-10. Facultatively
anaerobic. L-lactic acid is produced from glucose (TSYE broth + 5g glucose / L).
Isolated from the digestive tract of wasp (Vespula vulgaris), Modrany, Czech Republic.
Susceptible to some cephalosporines, macrolides and some penicillin-derived antibiotics. Resistant to aminoglycosides,
fluoroquinolones, sulphonamides, mupirocine and metronidazole.
- Killer, J., Svec, P., Sedlaccek, I., Cernohlavkova, J., Benada, O., Hroncova, Z., Havlik, J., Vlkova, E., Rada, V., Kopecny, J. and
Kofronova, O. 2014. Vagococcus entomophilus sp. nov., from the digestive tract of a wasp (Vespula vulgaris). Int. J. Syst. Evol.
Microbiol., 64, 731-737.
Some results are based on Biolog (GP2 MicroPlate), API 50 CH, Rapid ID 32 Strep
and API ZYM systems (bioMerieux).
Positive results for alpha-chymotrypsin, alpha-glucosidase, acid phosphatase, alkaline phosphatase, esterase (C4), esterase lipase
(C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase, arginine arylamidase, phenylalanine arylamidase, tyrosine
arylamidase, alanine arylamidase, glycine arylamidase, histidine arylamidase & serine arylamidase.
Can utilize: inulin, N-acetyl-D-glucosamine, amygdalin, arbutin, cellobiose, D-fructose, gentiobiose, alpha-D-glucose, D-mannose,
methyl-bets-D-glucoside, salicin, sucrose, trehalose, adenosine & uridine.
Negative results for arginine dihydrolase, cystine arylamidase, alpha- and beta-galactosidase, beta-glucosidase, alpha-
arabinosidase, beta-glucuronidase, alpha-mannosidase, N-acetyl-beta-glucosaminidase, nitrates reduction, pyroglutamic acid
arylamidase, beta-galactosidase-6-phosphate, alpha-fucosidase, proline arylamidase, leucyl glycine arylamidase, glutamyl glutamic
acid arylamidase, lipase (C14), valine arylamidase, trypsin, urease, catalase, oxidase, indole production, gelatin hydrolysis & Voges-
No utilization of: alpha- and beta-cyclodextrin, glycogen, mannan, Tween 40 and 80, L-arabinose, D-arabitol, L-fucose, D-galactose, D-
galacturonic acid, D-gluconic acid, myoinositol, alpha-lactose, lactulose, maltose, maltotriose, D-mannitol, melezitose, melibiose,
methyl-alpha-D-galactoside, methyl-beta-D-galactoside, 3-methyl glucose, methyl-alpha-D-glucoside, methyl-alpha-D-mannoside,
palatinose, D-psicose, raffinose, L-rhamnose, D-ribose, sedoheptulosan, D-sorbitol, stachyose, D-tagatose, turanose, xylitol, D-
xylose, acetic acid, alpha-, beta- and gamma-hydroxybutyric acid, p-hydroxyphenylacetic acid, alpha- and beta-ketoglutaric acid,
lactamide, D-lactic acid methyl ester, L-lactic acid, D-malic acid, L-malic acid, pyruvatic acid methyl ester, succinic acid monomethyl
ester, propionic acid, pyruvic acid, succinamic acid, succinic acid, N-acetyl-L-glutamic acid, L-alaninamide, D- and L-alanine, L-alanyl-
glycine, L-asparagine, L-glutamic acid, glycyl L-glutamic acid, L-pyroglutamic acid, L-serine, putrescine, 2,3-butanediol, glycerol, 29-
deoxyadenosine, inosine, thymidine, adenosine-59-monophosphate, thymidine-59-monophosphate, uridine-59-monophosphate,
D-fructose-6-phosphate, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate & DL-alpha-glycerol phosphate.
Dextrin utilization is variable.
(c) Costin Stoica